Tanghe Sofie, Cox Eric, Melkebeek Vesna, De Smet Stefaan, Millet Sam
Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Faculty of Bioscience Engineering, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium.
Laboratory of Immunology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Vet J. 2014 May;200(2):287-93. doi: 10.1016/j.tvjl.2014.02.025. Epub 2014 Feb 24.
This study investigated whether the immunocompetence of piglets at weaning was modulated by including different sources of n-3 polyunsaturated fatty acids (PUFA) in the maternal diet. From day 73 of gestation until weaning at 4 weeks, 32 pregnant sows were fed a palm oil-based diet (control group) or a diet including 1% linseed oil (C18:3n-3), 1% echium oil (C18:3n-3, C18:4n-3, C18:3n-6) or 1% fish oil (C20:5n-3, C22:6n-3). It was hypothesized that each diet would differently affect immune function through effects such as specific eicosanoid production. Piglets were fed a conventional diet without added n-3 PUFA from weaning until day 35 post-weaning. At weaning and 21 days post-weaning, four piglets per litter were immunized with bovine thyroglobulin. Blood samples were taken from weaning until day 35 post-weaning to determine thyroglobulin-specific antibodies, serum amyloid A (SAA) concentration and fatty acid composition. The fatty acid composition of the maternal diets was reflected in the plasma and red blood cells of the weaned piglets. The onset of the thyroglobulin-specific IgM response differed between dietary groups, with a delay in response for piglets from sows fed the fish oil diet. No significant dietary effects were observed on the thyroglobulin-specific IgG and IgA titres or on SAA concentrations in the piglet serum. Including n-3 PUFA in the maternal diet at the concentrations used in the present study had no major effects on the adaptive and innate immunity of the piglets after weaning.
本研究调查了在母猪日粮中添加不同来源的n-3多不饱和脂肪酸（PUFA）是否会调节断奶仔猪的免疫能力。从妊娠第73天到4周龄断奶，32头怀孕母猪被饲喂以棕榈油为基础的日粮（对照组）或包含1%亚麻籽油（C18:3n-3）、1%紫锥菊油（C18:3n-3、C18:4n-3、C18:3n-6）或1%鱼油（C20:5n-3、C22:6n-3）的日粮。研究假设每种日粮会通过特定类二十烷酸生成等效应不同地影响免疫功能。从断奶到断奶后第35天，给仔猪饲喂不添加n-3 PUFA的常规日粮。在断奶时和断奶后21天，每窝选取4头仔猪用牛甲状腺球蛋白进行免疫。从断奶到断奶后第35天采集血样，以测定甲状腺球蛋白特异性抗体、血清淀粉样蛋白A（SAA）浓度和脂肪酸组成。断奶仔猪的血浆和红细胞中反映了母猪日粮的脂肪酸组成。日粮组之间甲状腺球蛋白特异性IgM反应的起始时间不同，饲喂鱼油日粮的母猪所产仔猪的反应延迟。在仔猪血清中，未观察到日粮对甲状腺球蛋白特异性IgG和IgA滴度或SAA浓度有显著影响。在本研究使用的浓度下，在母猪日粮中添加n-3 PUFA对断奶后仔猪的适应性免疫和先天性免疫没有重大影响。
