成年果蝇胃肠道的整体免疫染色。
Whole-mount immunostaining of the adult Drosophila gastrointestinal tract.
作者信息
Micchelli Craig A
机构信息
Department of Developmental Biology, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, United States.
出版信息
Methods. 2014 Jun 15;68(1):273-9. doi: 10.1016/j.ymeth.2014.03.022. Epub 2014 Mar 27.
Abstract
The gastrointestinal (GI) tract harbors an essential barrier epithelium that separates an organism from its changing external environment. As such, the gut epithelium is a fascinating nexus of stem cell biology, immunology and physiology. Investigators have sought to mine this rich interface for new biological and mechanistic insights. Many of the powerful genetic approaches developed in Drosophila have proven effective in the study of the gut. The goal of this article is to present a method for dissecting, immunostaining and mounting samples of the adult Drosophila GI tract. This protocol combines readily with techniques to label cell lineages and/or challenge the system with environmental perturbations, which are briefly discussed.
摘要
胃肠道拥有一层重要的屏障上皮，将生物体与其不断变化的外部环境分隔开来。因此，肠道上皮是干细胞生物学、免疫学和生理学的一个迷人交汇点。研究人员一直在探索这个丰富的界面，以获取新的生物学和机制见解。许多在果蝇中开发的强大遗传方法已被证明在肠道研究中有效。本文的目的是介绍一种解剖、免疫染色和固定成年果蝇胃肠道样本的方法。该方案可轻松与标记细胞谱系和/或用环境扰动挑战该系统的技术相结合，本文将简要讨论这些技术。
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