在成年果蝇的中肠后部，肠内分泌细胞由干细胞通过一种独特的祖细胞产生。
Enteroendocrine cells are generated from stem cells through a distinct progenitor in the adult Drosophila posterior midgut.
作者信息
Zeng Xiankun, Hou Steven X
机构信息
Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21701, USA
出版信息
Development. 2015 Feb 15;142(4):644-53. doi: 10.1242/dev.113357.
Functional mature cells are continually replenished by stem cells to maintain tissue homoeostasis. In the adult Drosophila posterior midgut, both terminally differentiated enterocyte (EC) and enteroendocrine (EE) cells are generated from an intestinal stem cell (ISC). However, it is not clear how the two differentiated cells are generated from the ISC. In this study, we found that only ECs are generated through the Su(H)GBE(+) immature progenitor enteroblasts (EBs), whereas EEs are generated from ISCs through a distinct progenitor pre-EE by a novel lineage-tracing system. EEs can be generated from ISCs in three ways: an ISC becoming an EE, an ISC becoming a new ISC and an EE through asymmetric division, or an ISC becoming two EEs through symmetric division. We further identified that the transcriptional factor Prospero (Pros) regulates ISC commitment to EEs. Our data provide direct evidence that different differentiated cells are generated by different modes of stem cell lineage specification within the same tissues.
功能成熟的细胞不断由干细胞补充，以维持组织稳态。在成年果蝇的中肠后部，终末分化的肠上皮细胞（EC）和肠内分泌细胞（EE）均由肠道干细胞（ISC）产生。然而，尚不清楚这两种分化细胞是如何从ISC产生的。在本研究中，我们发现只有EC是通过Su(H)GBE(+)未成熟祖细胞成肠细胞（EB）产生的，而EE是通过一种独特的祖细胞前EE，利用一种新的谱系追踪系统从ISC产生的。EE可以通过三种方式从ISC产生：一个ISC变成一个EE，一个ISC通过不对称分裂变成一个新的ISC和一个EE，或者一个ISC通过对称分裂变成两个EE。我们进一步确定转录因子Prospero（Pros）调节ISC向EE的分化。我们的数据提供了直接证据，表明同一组织内不同的分化细胞是由干细胞谱系特化的不同模式产生的。
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