Stubbs Keith A
School of Chemistry and Biochemistry, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
Carbohydr Res. 2014 May 22;390:9-19. doi: 10.1016/j.carres.2014.02.023. Epub 2014 Mar 4.
Carbohydrate-processing enzymes are gaining more attention due to their roles in health and disease as these enzymes are involved in the construction and deconstruction of vast arrays of glycan structures. As a result, the development of methods to identify these enzymes in complex biological systems is of increasing interest. Activity-based proteomics probes (ABPPs) are increasingly being used in glycobiology to detect and identify functionally related proteins (and homologues) within a biological system. This review will describe the design of activity-based proteomics probes, provide examples of compounds that have been used to profile activity in the area of carbohydrate-processing enzymes, and give some future perspectives.
碳水化合物加工酶正受到越来越多的关注，因为它们在健康和疾病中发挥着作用，这些酶参与了大量聚糖结构的构建和解构。因此，开发在复杂生物系统中鉴定这些酶的方法越来越受到关注。基于活性的蛋白质组学探针（ABPPs）越来越多地用于糖生物学中，以检测和鉴定生物系统中功能相关的蛋白质（及其同源物）。本综述将描述基于活性的蛋白质组学探针的设计，提供已用于分析碳水化合物加工酶领域活性的化合物实例，并给出一些未来展望。
