Liu Yun, Fredrickson James K, Sadler Natalie C, Nandhikonda Premchendar, Smith Richard D, Wright Aaron T
Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, 100029 Beijing, China.
Biological Sciences Division, Pacific Northwest National Laboratory, 902 Battelle Blvd, MSIN J4-02, Box 999, Richland, WA 99352 USA.
Biotechnol Biofuels. 2015 Sep 25;8:156. doi: 10.1186/s13068-015-0343-7. eCollection 2015.
The development of renewable biofuels is a global priority, but success will require novel technologies that greatly improve our understanding of microbial systems biology. An approach with great promise in enabling functional characterization of microbes is activity-based protein profiling (ABPP), which employs chemical probes to directly measure enzyme function in discrete enzyme classes in vivo and/or in vitro, thereby facilitating the rapid discovery of new biocatalysts and enabling much improved biofuel production platforms. We review general design strategies in ABPP, and highlight recent advances that are or could be pivotal to biofuels processes including applications of ABPP to cellulosic bioethanol, biodiesel, and phototrophic production of hydrocarbons. We also examine the key challenges and opportunities of ABPP in renewable biofuels research. The integration of ABPP with molecular and systems biology approaches will shed new insight on the catalytic and regulatory mechanisms of functional enzymes and their synergistic effects in the field of biofuels production.
可再生生物燃料的开发是全球优先事项，但要取得成功需要新技术，这些技术能极大地增进我们对微生物系统生物学的理解。基于活性的蛋白质谱分析（ABPP）是一种在实现微生物功能表征方面极具前景的方法，它利用化学探针直接在体内和/或体外测量离散酶类中的酶功能，从而促进新生物催化剂的快速发现，并实现大幅改进的生物燃料生产平台。我们综述了ABPP的一般设计策略，并强调了近期对生物燃料生产过程至关重要或可能至关重要的进展，包括ABPP在纤维素生物乙醇、生物柴油和光合烃生产中的应用。我们还探讨了ABPP在可再生生物燃料研究中的关键挑战和机遇。将ABPP与分子和系统生物学方法相结合，将为生物燃料生产领域中功能酶的催化和调控机制及其协同效应带来新的见解。
