Brezezińska-Błaszczyk E, Czuwaj M, Wyczółkowska J
Department of Biogenic Amines, Polish Academy of Sciences, Lódź.
Arch Immunol Ther Exp (Warsz). 1987;35(6):871-6.
The mesenteric and pulmonary mast cells of guinea pigs were obtained by enzymatic dispersion of the tissues with the enzyme collagenase. The guinea pig mast cells obtained by this method were morphologically intact, as judged by light microscopy. The mast cells from lung and mesentery of actively sensitized guinea pigs released histamine in a dose-dependent fashion after challenge with specific antigen. Both pulmonary and mesenteric mast cells of nonsensitized animals were unresponsive to the action of compound 48/80 and concanavalin A. Both populations of mast cells responded to polymyxin B, but the magnitude of histamine release was low. These cells responded strongly to the ionophore A23187; the mesenteric mast cells were markedly more reactive than pulmonary mast cells.
通过用胶原酶对组织进行酶解分散，获得豚鼠的肠系膜和肺肥大细胞。通过这种方法获得的豚鼠肥大细胞在光学显微镜下判断形态完整。主动致敏豚鼠的肺和肠系膜肥大细胞在用特异性抗原激发后以剂量依赖方式释放组胺。未致敏动物的肺和肠系膜肥大细胞对化合物48/80和伴刀豆球蛋白A的作用无反应。两种肥大细胞群体对多粘菌素B均有反应，但组胺释放量较低。这些细胞对离子载体A23187反应强烈；肠系膜肥大细胞的反应性明显高于肺肥大细胞。
