a Department of Dental Materials and Prosthodontics, Araraquara Dental School , Universidade Estadual Paulista, UNESP , São Paulo , Brazil.
Biofouling. 2014;30(5):525-33. doi: 10.1080/08927014.2014.894028. Epub 2014 Apr 1.
This study evaluated the effect of experimental coatings, containing zwitterion or hydrophilic monomers, on the adherence of Candida albicans, Candida glabrata, and Streptococcus mutans to an acrylic resin. Acrylic samples (smooth or rough surfaces) were left untreated (control) or coated with one of the following experimental coatings: 3-hydroxypropylmethacrylate (HP) or sulfobetaine methacrylate (S), at concentrations of 25, 30, or 35%. Half of the specimens were coated with saliva. The adhesion test was performed by incubating specimens in C. albicans, C. glabrata, and S. mutans suspensions at 37°C for 90 min. The number of adhered microorganisms was determined by metabolic activity (XTT) and by cell viability (CFU). All coated specimens exhibited lower absorbance and CFU values compared to control specimens. Saliva and roughness did not promote microorganism adherence. An XPS analysis confirmed the modification in the chemical composition of the coatings in the experimental samples. These experimental coatings significantly reduced the adherence of C. albicans, C. glabrata and S. mutans to acrylic resin.
本研究评估了含两性离子或亲水性单体的实验涂层对白色念珠菌、光滑念珠菌和变异链球菌在丙烯酸树脂上黏附的影响。丙烯酸样本（光滑或粗糙表面）未经处理（对照）或用以下实验涂层之一进行涂层：3-羟丙基甲基丙烯酸酯（HP）或磺基甜菜碱甲基丙烯酸酯（S），浓度为 25、30 或 35%。一半的标本涂有唾液。黏附试验通过将标本在 37°C 的白色念珠菌、光滑念珠菌和变异链球菌悬浮液中孵育 90 分钟来进行。通过代谢活性（XTT）和细胞活力（CFU）来确定黏附的微生物数量。与对照标本相比，所有涂层标本的吸光度和 CFU 值均较低。唾液和粗糙度并没有促进微生物的黏附。XPS 分析证实了实验样本中涂层化学成分的修饰。这些实验涂层显著降低了白色念珠菌、光滑念珠菌和变异链球菌在丙烯酸树脂上的黏附。
