Shinada K, Ozaki F, Cordiero J G, Okada S, Shimoyama K, Nagao M, Ichinose S, Yamashita Y
Department of Preventive Dentistry, Faculty of Dentistry, Tokyo Medical and Dental University.
Kokubyo Gakkai Zasshi. 1995 Jun;62(2):281-6. doi: 10.5357/koubyou.62.281.
Denture stomatitis is caused mainly by infection of Candida albicans and Streptococcus mutans, the most predominant members of the bacterial flora on denture surfaces. The purpose of this study was to investigate the adhesion in vitro of C. albicans and of S. mutans to acrylic surfaces and their interactions. Concentrations of C. albicans and of S. mutans (mutational phase I and III in which activities of glucosyltransferases are higher and lower respectively) were of high levels (> or = 5.0 x 107 colony-forming-units per ml; CFU/ml). The colonies in brain-heart-infusion (BHI) broth added 5% sucrose incubated at 37 degrees C for 18 and 72 hours. Then the samples were dried and evaporated, and examined by S-700 and S-4100 SEM (HITACHI). It was found that S. mutans (mutational phase I) synthesized water-insoluble glucans concomitantly with the adhesion of both S. mutans and C. albicans to the acrylic surfaces. Also, adhesion of C. albicans, mediated by cell-to-cell interactions with S. mutans (especially mutational phase III), to the surfaces was observed.
义齿性口炎主要由白色念珠菌和变形链球菌感染引起，这两种菌是义齿表面细菌群落中最主要的成员。本研究的目的是调查白色念珠菌和变形链球菌在体外对丙烯酸表面的黏附情况及其相互作用。白色念珠菌和变形链球菌（分别处于葡糖基转移酶活性较高和较低的突变I期和III期）的浓度处于高水平（≥5.0×10⁷ 菌落形成单位每毫升；CFU/ml）。将其接种于添加5%蔗糖的脑心浸液（BHI）肉汤中，在37℃孵育18小时和72小时。然后将样品干燥并蒸发，用日立S-700和S-4100扫描电子显微镜进行检测。结果发现，变形链球菌（突变I期）在变形链球菌和白色念珠菌黏附于丙烯酸表面的同时合成了水不溶性葡聚糖。此外，还观察到白色念珠菌通过与变形链球菌（尤其是突变III期）的细胞间相互作用介导而黏附于表面。
