A simple, sensitive method of analyzing bacterial ribosomal DNA polymorphism.
作者信息
Picard-Pasquier N, Ouagued M, Picard B, Goullet P, Krishnamoorthy R
机构信息
Institut de Pathologie Moléculaire, Institut National de la Santé et de la Recherche Médicale, Paris, France.
出版信息
Electrophoresis. 1989 Mar;10(3):186-9. doi: 10.1002/elps.1150100306.
PMID:2468482
Abstract
A rapid DNA extraction procedure and random primer labelling of Escherichia coli ribosomal RNA with cloned reverse transcriptase have been used to establish a simple and highly sensitive method for studying ribosomal DNA polymorphism in bacteria. Examples of inter- and intraspecies differentiation of bacteria are given and potential applications in bacterial epidemiology and taxonomy are discussed.
摘要
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