van der Krol A R, Mol J N, Stuitje A R
Department of Applied Genetics, Free University, Amsterdam, The Netherlands.
Gene. 1988 Dec 10;72(1-2):45-50. doi: 10.1016/0378-1119(88)90126-6.
Plants are the first multicellular higher eukaryotic organisms in which artificial antisense genes have been shown to down-regulate target gene expression. Manipulations with an antisense gene can serve as a tool to study the effect of a particular plant gene inactivation, the interaction of gene products whose genes are coordinately expressed, or the functional analysis of cryptic genes. Transgenic plants harbouring an antisense gene already gave rise to patentable new characteristics, showing that the technique has great scientific and economic value.
植物是首个被证明人工反义基因可下调靶基因表达的多细胞高等真核生物。利用反义基因进行操作可作为一种工具,用于研究特定植物基因失活的影响、基因协调表达的基因产物之间的相互作用,或隐匿基因的功能分析。携带反义基因的转基因植物已经产生了可申请专利的新特性,这表明该技术具有巨大的科学和经济价值。