Hadden J W, Caspritz G, Zheng Q Y, Chen H, Wolstencroft R, Hadden E M
Program of Immunopharmacology, University of South Florida Medical College, Tampa 33612.
Int J Immunopharmacol. 1989;11(1):13-9. doi: 10.1016/0192-0561(89)90094-5.
Previous reports suggest that immunotherapy can induce T-cell development in athymic nude mice. Eight-week-old BALB/c nu/nu (athymic nude) mice were treated for 3, 6 and 12 weeks with thymosin fraction 5 (TF-5), buffy coat interleukins (BC-IL), recombinant IL-2 (rIL-2), a combination of TF-5 and BC-IL, isoprinosine or imuthiol. Control animals were treated with sterile saline or were given a syngeneic thymus graft. Spleen weight, cell yields and Thy 1.2+ T-cell markers were monitored and spleen cell proliferative responses to rIL-2, concanavalin A (Con A), Con A + rIL-2, phytohemagglutinin (PHA) and endotoxin (LPS) were assessed. Only mice transplanted with a syngeneic thymus showed progressive increases in both T-cell number and function. Minor changes in proliferative responses were noted at 3 weeks with all treatments; however, no biologically significant reconstitution of T-cell number or function was observed.
先前的报告表明免疫疗法可在无胸腺裸鼠中诱导T细胞发育。对8周龄的BALB/c nu/nu（无胸腺裸）小鼠用胸腺素组分5（TF-5）、血沉棕黄层白细胞介素（BC-IL）、重组白细胞介素-2（rIL-2）、TF-5与BC-IL的组合、异丙肌苷或硫唑嘌呤治疗3周、6周和12周。对照动物用无菌生理盐水治疗或接受同基因胸腺移植。监测脾脏重量、细胞产量和Thy 1.2+ T细胞标志物，并评估脾细胞对rIL-2、刀豆球蛋白A（Con A）、Con A+rIL-2、植物血凝素（PHA）和内毒素（LPS）的增殖反应。只有移植了同基因胸腺的小鼠T细胞数量和功能均出现逐渐增加。所有治疗在3周时均观察到增殖反应有轻微变化；然而，未观察到T细胞数量或功能有生物学意义的重建。
