Gluck Jessica M, Delman Connor, Chyu Jennifer, MacLellan W Robb, Shemin Richard J, Heydarkhan-Hagvall Sepideh
Department of Surgery, Cardiovascular Tissue Engineering Laboratory, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California; Department of Medicine, Cardiovascular Research Laboratories, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California.
J Biomed Mater Res B Appl Biomater. 2014 Nov;102(8):1730-9. doi: 10.1002/jbm.b.33155. Epub 2014 Mar 31.
We examined the effects of the microenvironment on vascular differentiation of murine cardiovascular progenitor cells (CPCs). We isolated CPCs and seeded them in culture exposed to the various extracellular matrix (ECM) proteins in both two-dimensional (2D) and 3D culture systems. To better understand the contribution of the microenvironment to vascular differentiation, we analyzed endothelial and smooth muscle cell differentiation at both day 7 and day 14. We found that laminin and vitronectin enhanced vascular endothelial cell differentiation while fibronectin enhanced vascular smooth muscle cell differentiation. We also observed that the effects of the 3D electrospun scaffolds were delayed and not noticeable until the later time point (day 14), which may be due to the amount of time necessary for the cells to migrate to the interior of the scaffold. The study characterized the contributions of both ECM proteins and the addition of a 3D culture system to continued vascular differentiation. Additionally, we demonstrated the capability bioengineer a CPC-derived vascular graft.
我们研究了微环境对小鼠心血管祖细胞（CPCs）血管分化的影响。我们分离出CPCs，并将它们接种到二维（2D）和三维（3D）培养系统中，使其暴露于各种细胞外基质（ECM）蛋白中。为了更好地理解微环境对血管分化的作用，我们在第7天和第14天分析了内皮细胞和平滑肌细胞的分化情况。我们发现层粘连蛋白和玻连蛋白可增强血管内皮细胞分化，而纤连蛋白可增强血管平滑肌细胞分化。我们还观察到，3D电纺支架的作用延迟，直到较晚时间点（第14天）才明显，这可能是由于细胞迁移到支架内部所需的时间。该研究描述了ECM蛋白和添加3D培养系统对持续血管分化的作用。此外，我们展示了生物工程构建CPC来源血管移植物的能力。
