巨噬细胞移动抑制因子对兔准分子原位角膜磨镶术后角膜敏感性的影响

Effect of macrophage migration inhibitory factor on corneal sensitivity after laser in situ keratomileusis in rabbit.

作者信息

Hyon Joon Young, Hose Stacey, Gongora Celine, Sinha Debasish, O'Brien Terrence

机构信息

Department of Ophthalmology, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Korea.

Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

出版信息

Korean J Ophthalmol. 2014 Apr;28(2):170-6. doi: 10.3341/kjo.2014.28.2.170. Epub 2014 Mar 14.

DOI:10.3341/kjo.2014.28.2.170

PMID:24688261

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3958634/

Abstract

PURPOSE

To investigate the effect of macrophage migration inhibitory factor (MIF) on corneal sensitivity after laser in situ keratomileusis (LASIK) surgery.

METHODS

New Zealand white rabbits were used in this study. A hinged corneal flap (160-µm thick) was created with a microkeratome, and -3.0 diopter excimer laser ablation was performed. Expressions of MIF mRNA in the corneal epithelial cells and surrounding inflammatory cells were analyzed using reverse transcription polymerase chain reaction at 48 hours after LASIK. After LASIK surgery, the rabbits were topically given either 1) a balanced salt solution (BSS), 2) MIF (100 ng/mL) alone, or 3) a combination of nerve growth factor (NGF, 100 ug/mL), neurotrophine-3 (NT-3, 100 ng/mL), interleukin-6 (IL-6, 5 ng/mL), and leukemia inhibitory factor (LIF, 5 ng/mL) four times a day for three days. Preoperative and postoperative corneal sensitivity at two weeks and at 10 weeks were assessed using the Cochet-Bonnet esthesiometer.

RESULTS

Expression of MIF mRNA was 2.5-fold upregulated in the corneal epithelium and 1.5-fold upregulated in the surrounding inflammatory cells as compared with the control eyes. Preoperative baseline corneal sensitivity was 40.56 ± 2.36 mm. At two weeks after LASIK, corneal sensitivity was 9.17 ± 5.57 mm in the BSS treated group, 21.92 ± 2.44 mm in the MIF treated group, and 22.42 ± 1.59 mm in the neuronal growth factors-treated group (MIF vs. BSS, p < 0.0001; neuronal growth factors vs. BSS, p < 0.0001; MIF vs. neuronal growth factors, p = 0.815). At 10 weeks after LASIK, corneal sensitivity was 15.00 ± 9.65, 35.00 ± 5.48, and 29.58 ± 4.31 mm respectively (MIF vs. BSS, p = 0.0001; neuronal growth factors vs. BSS, p = 0.002; MIF vs. neuronal growth factors, p = 0.192). Treatment with MIF alone could achieve as much of an effect on recovery of corneal sensation as treatment with combination of NGF, NT-3, IL-6, and LIF.

CONCLUSIONS

Topically administered MIF plays a significant role in the early recovery of corneal sensitivity after LASIK in the experimental animal model.

摘要

目的

探讨巨噬细胞移动抑制因子（MIF）对准分子激光原位角膜磨镶术（LASIK）术后角膜敏感性的影响。

方法

本研究使用新西兰白兔。用微型角膜刀制作一个带蒂角膜瓣（厚160μm），并进行-3.0屈光度的准分子激光消融。在LASIK术后48小时，使用逆转录聚合酶链反应分析角膜上皮细胞和周围炎症细胞中MIF mRNA的表达。LASIK术后，兔子局部给予以下处理：1）平衡盐溶液（BSS）；2）单独的MIF（100 ng/mL）；3）神经生长因子（NGF，100μg/mL）、神经营养因子-3（NT-3，100 ng/mL）、白细胞介素-6（IL-6，5 ng/mL）和白血病抑制因子（LIF，5 ng/mL）的组合，每天4次，共3天。使用Cochet-Bonnet眼压计评估术前以及术后2周和10周时的角膜敏感性。

结果

与对照眼相比，角膜上皮中MIF mRNA的表达上调了2.5倍，周围炎症细胞中上调了1.5倍。术前基线角膜敏感性为40.56±2.36 mm。LASIK术后2周，BSS处理组的角膜敏感性为9.17±5.57 mm，MIF处理组为21.92±2.44 mm，神经生长因子处理组为22.42±1.59 mm（MIF与BSS相比，p<0.0001；神经生长因子与BSS相比，p<0.0001；MIF与神经生长因子相比，p = 0.815）。LASIK术后10周，角膜敏感性分别为15.00±9.65、35.00±5.48和29.58±4.31 mm（MIF与BSS相比，p = 0.0001；神经生长因子与BSS相比，p = 0.002；MIF与神经生长因子相比，p = 0.192）。单独使用MIF治疗对角膜感觉恢复的效果与使用NGF、NT-3、IL-6和LIF组合治疗的效果相当。