Amorim Irina, Freitas Daniela P, Magalhães Ana, Faria Fátima, Lopes Célia, Faustino Augusto M, Smet Annemieke, Haesebrouck Freddy, Reis Celso A, Gärtner Fátima
Department of Pathology and Molecular Immunology of the Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Rua Jorge Viterbo Ferreira nr. 228, Porto, 4050-313, Portugal; Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Rua Dr. Roberto Frias s/n, Porto, 4200-465, Portugal.
Helicobacter. 2014 Aug;19(4):249-59. doi: 10.1111/hel.12125. Epub 2014 Apr 2.
The gastric mucosa of dogs is often colonized by non-Helicobacter pylori helicobacters (NHPH), while H. pylori is the predominant gastric Helicobacter species in humans. The colonization of the human gastric mucosa by H. pylori is highly dependent on the recognition of host glycan receptors. Our goal was to define the canine gastric mucosa glycophenotype and to evaluate the capacity of different gastric Helicobacter species to adhere to the canine gastric mucosa.
The glycosylation profile in body and antral compartments of the canine gastric mucosa, with focus on the expression of histo-blood group antigens was evaluated. The in vitro binding capacity of FITC-labeled H. pylori and NHPH to the canine gastric mucosa was assessed in cases representative of the canine glycosylation pattern.
The canine gastric mucosa lacks expression of type 1 Lewis antigens and presents a broad expression of type 2 structures and A antigen, both in the surface and glandular epithelium. Regarding the canine antral mucosa, H. heilmannii s.s. presented the highest adhesion score whereas in the body region the SabA-positive H. pylori strain was the strain that adhered more.
The canine gastric mucosa showed a glycosylation profile different from the human gastric mucosa suggesting that alternative glycan receptors may be involved in Helicobacter spp. binding. Helicobacter pylori and NHPH strains differ in their ability to adhere to canine gastric mucosa. Among the NHPH, H. heilmannii s.s. presented the highest adhesion capacity in agreement with its reported colonization of the canine stomach.
犬的胃黏膜常被非幽门螺杆菌属螺旋杆菌（NHPH）定植，而幽门螺杆菌是人类胃中主要的螺旋杆菌种类。幽门螺杆菌对人类胃黏膜的定植高度依赖于对宿主聚糖受体的识别。我们的目标是确定犬胃黏膜的糖表型，并评估不同胃螺旋杆菌种类黏附犬胃黏膜的能力。
评估犬胃黏膜体部和窦部的糖基化谱，重点关注组织血型抗原的表达。在具有犬糖基化模式代表性的病例中，评估异硫氰酸荧光素标记的幽门螺杆菌和NHPH对犬胃黏膜的体外结合能力。
犬胃黏膜缺乏1型Lewis抗原的表达，在表面和腺上皮中均广泛表达2型结构和A抗原。关于犬窦黏膜，海氏螺杆菌呈现出最高的黏附评分，而在体部区域，SabA阳性的幽门螺杆菌菌株是黏附性更强的菌株。
犬胃黏膜显示出与人类胃黏膜不同的糖基化谱，这表明可能有其他聚糖受体参与螺旋杆菌属的结合。幽门螺杆菌和NHPH菌株在黏附犬胃黏膜的能力上存在差异。在NHPH中，海氏螺杆菌的黏附能力最高，这与其报道的在犬胃中的定植情况一致。
