两个可变表面糖蛋白基因可区分布氏冈比亚锥虫的不同亚株。

Two variant surface glycoprotein genes distinguish between different substrains of Trypanosoma brucei gambiense.

作者信息

Barnes D A, Mottram J, Selkirk M, Agabian N

机构信息

Molecular Parasitology-Intercampus Program, School of Pharmacy, University of California, San Francisco.

出版信息

Mol Biochem Parasitol. 1989 May 1;34(2):135-46. doi: 10.1016/0166-6851(89)90005-4.

DOI:10.1016/0166-6851(89)90005-4

PMID:2469013

Abstract

Trypanosoma brucei gambiense differs from other T. brucei subspecies in the stability and conservation of its bloodstream form antigenic repertoire. Two variant surface glycoprotein (VSG) cDNA clones corresponding to the antigens U1 and L2 were isolated from T. b. gambiense bacteriophage lambda gt11 expression libraries and characterized. A third VSG cDNA clone, P1, was also examined. The L2 and U1 VSG genes are present in a large number of T. b. gambiense stocks isolated over a thirty-year period from different geographical areas of Africa, suggesting that they are stably maintained in the T. b. gambiense genome. These probes may be useful in epidemiological studies of Gambian sleeping sickness to differentiate between T. b. gambiense isolates.

摘要

布氏冈比亚锥虫在其血流形式抗原库的稳定性和保守性方面与其他布氏锥虫亚种不同。从布氏冈比亚锥虫噬菌体λgt11表达文库中分离并鉴定了两个与抗原U1和L2相对应的可变表面糖蛋白（VSG）cDNA克隆。还检测了第三个VSG cDNA克隆P1。L2和U1 VSG基因存在于从非洲不同地理区域分离的大量布氏冈比亚锥虫种群中，这些种群跨越三十年，这表明它们在布氏冈比亚锥虫基因组中稳定存在。这些探针可能有助于冈比亚昏睡病的流行病学研究，以区分布氏冈比亚锥虫分离株。