Hou Steven S, Rice William L, Bacskai Brian J, Kumar Anand T N
Opt Lett. 2014 Mar 1;39(5):1165-8. doi: 10.1364/OL.39.001165.
We present a novel, hybrid approach for time domain fluorescence tomography that efficiently combines lifetime multiplexing using late-arriving or asymptotic photons, with the high spatial resolution capability of early photon tomography. We also show that a decay amplitude-based asymptotic approach is superior to direct inversion of late-arriving photons for tomographic lifetime imaging within turbid media. The hybrid reconstruction approach is experimentally shown to recover fluorescent inclusions separated as close as 1.4 mm, with improved resolution and reduced cross talk compared to just using early photons or the asymptotic approach alone.
我们提出了一种用于时域荧光层析成像的新型混合方法，该方法有效地将利用晚到或渐近光子的寿命复用与早期光子层析成像的高空间分辨率能力相结合。我们还表明，对于浑浊介质中的层析寿命成像，基于衰减幅度的渐近方法优于直接对晚到光子进行反演。实验表明，与仅使用早期光子或单独使用渐近方法相比，这种混合重建方法能够恢复间距小至1.4毫米的荧光内含物，具有更高的分辨率和更低的串扰。
