Tian Lei, Wang Jingyan, Waller Laura
Opt Lett. 2014 Mar 1;39(5):1326-9. doi: 10.1364/OL.39.001326.
We demonstrate 3D differential phase-contrast (DPC) microscopy, based on computational illumination with a programmable LED array. By capturing intensity images with various illumination angles generated by sequentially patterning an LED array source, we digitally refocus images through various depths via light field processing. The intensity differences from images taken at complementary illumination angles are then used to generate DPC images, which are related to the gradient of phase. The proposed method achieves 3D DPC with simple, inexpensive optics and no moving parts. We experimentally demonstrate our method by imaging a camel hair sample in 3D.
我们展示了基于可编程LED阵列的计算照明的三维差分相衬（DPC）显微镜。通过捕获由LED阵列光源顺序图案化产生的各种照明角度下的强度图像，我们通过光场处理在不同深度对图像进行数字重新聚焦。然后，利用在互补照明角度下拍摄的图像的强度差异来生成与相位梯度相关的DPC图像。所提出的方法通过简单、廉价的光学器件且无需移动部件即可实现三维DPC。我们通过对骆驼毛样本进行三维成像，实验验证了我们的方法。
