Ker Yaw-Bee, Peng Chiung-Chi, Chang Wan-Lin, Chyau Charng-Cherng, Peng Robert Y
Department of Applied Food Technology, Hungkuang University, Taichung, Taiwan, ROC.
Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan, ROC.
PLoS One. 2014 Apr 1;9(4):e93191. doi: 10.1371/journal.pone.0093191. eCollection 2014.
Antrodan, a protein-bound polysaccharide isolated from Antrodia cinnamomea mycelia, was demonstrated to exhibit significant anti-inflammatory bioactivity in vitro. However, its role in hepatic injury in vivo still remains unclear. We hypothesized that antrodan may have beneficial hepatoprotective effects. To verify this, a lipopolysaccharide (LPS)-Sprague-Dawley rat model was used. Antrodan protected against liver damage by suppressing LPS-stimulated serum glutamine-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), interleukin (IL)-6, hepatic thiobarbituric acid reactive substances (TBARS), nitric oxide (NO), inducible NO synthase (iNOS) and nuclear factor (NF)-κB, and by effectively alleviating the downregulated hepatic superoxide dismutase (SOD), catalase, and glutathione peroxidase (GSH-Px). Hematoxylin-eosin staining revealed that antrodan at a dosage of 40 mg/kg was able to alleviate LPS-induced liver damage to a normal status. In addition, we identified the partial main architectural backbone of antrodan to have a 1 → 3 linear β-glycosidic backbone of mannan linked by β-1 → 3 glucosidic branches. Conclusively, antrodan can potentially ameliorate liver damage in vivo by suppressing oxidative stress induced by LPS.
安络小皮伞菌多糖（Antrodan）是一种从樟芝菌丝体中分离得到的蛋白结合多糖，已证实在体外具有显著的抗炎生物活性。然而，其在体内肝损伤中的作用仍不清楚。我们推测安络小皮伞菌多糖可能具有有益的肝脏保护作用。为了验证这一点，使用了脂多糖（LPS）-斯普拉格-道利大鼠模型。安络小皮伞菌多糖通过抑制LPS刺激的血清谷草转氨酶（GOT）、谷丙转氨酶（GPT）、白细胞介素（IL）-6、肝硫代巴比妥酸反应性物质（TBARS）、一氧化氮（NO）、诱导型一氧化氮合酶（iNOS）和核因子（NF）-κB，以及有效缓解肝脏中超氧化物歧化酶（SOD）、过氧化氢酶和谷胱甘肽过氧化物酶（GSH-Px）的下调来保护肝脏免受损伤。苏木精-伊红染色显示，40mg/kg剂量的安络小皮伞菌多糖能够将LPS诱导的肝损伤减轻至正常状态。此外，我们确定安络小皮伞菌多糖的部分主要结构骨架具有由β-1→3糖苷分支连接的甘露聚糖1→3线性β-糖苷骨架。总之，安络小皮伞菌多糖可能通过抑制LPS诱导的氧化应激来改善体内肝损伤。
