Zingue Stéphane, Njamen Dieudonné, Mvondo Marie Alfrede, Magne Nde Chantal Beatrice
J Complement Integr Med. 2014 Jun;11(2):83-92. doi: 10.1515/jcim-2013-0049.
Millettia macrophylla Benth is a Cameroonian medicinal plant traditionally used to alleviate menopause-related problems. The methanol soluble fraction of this plant was shown to exhibit estrogenic effects in vitro in Human Embryonic kidney cells, and in vivo on ovariectomized rat following the classical uterotrophic assay. Since estrogens have been involved in bone remodeling process, the present study then aimed at evaluating bone loss preventive effects of the methanol soluble fraction of Millettia macrophylla (MM-met) in ovariectomized rat model.
Twenty-five healthy Wistar female rats (3-month-old) were randomly assigned to a sham-operated group and to four treated ovariectomized (OVX) groups. Treatments lasted 8 weeks and animals were sacrificed. The uterus, the femoral and the tibia bones of each animal were collected, weighed and fixed in 10% formalin for histological analysis.
Results showed that ovariectomy decreased uterine wet weight (p<0.01), induced body weight gain (p<0.01), decreased both femoral and tibia bone density and mineral content and increased alkaline phosphatase activity (p<0.05). E2V and MM-met treatments in general prevented bone mass loss and/or bone density loss. At all tested doses, MM-met induced a significant decrease of alkaline phosphatase activity (p<0.05). As observed with E2V, MM-met also induced a significant protective effect on bone, and this was indicated by an abundance of bone marrow in an almost intact trabecular network.
The overall results show that the methanol soluble fraction of Millettia macrophylla may prevent ovariectomy-induced bone mass loss and deterioration of the trabecular microarchitecture.
大叶崖豆藤是喀麦隆的一种药用植物，传统上用于缓解与更年期相关的问题。该植物的甲醇可溶部分在体外人胚肾细胞中显示出雌激素样作用，并在经典子宫增重试验中对去卵巢大鼠显示出体内雌激素样作用。由于雌激素参与骨重塑过程，因此本研究旨在评估大叶崖豆藤甲醇可溶部分（MM-met）对去卵巢大鼠模型骨丢失的预防作用。
将25只健康的3月龄Wistar雌性大鼠随机分为假手术组和4个去卵巢（OVX）治疗组。治疗持续8周，然后处死动物。收集每只动物的子宫、股骨和胫骨，称重并固定于10%福尔马林中用于组织学分析。
结果显示，去卵巢降低了子宫湿重（p<0.01），导致体重增加（p<0.01），降低了股骨和胫骨的骨密度及矿物质含量，并增加了碱性磷酸酶活性（p<0.05）。E2V和MM-met治疗总体上预防了骨量丢失和/或骨密度丢失。在所有测试剂量下，MM-met均显著降低了碱性磷酸酶活性（p<0.05）。与E2V观察到的情况一样，MM-met对骨骼也有显著的保护作用，这表现为在几乎完整的小梁网络中有丰富的骨髓。
总体结果表明，大叶崖豆藤的甲醇可溶部分可能预防去卵巢诱导的骨量丢失和小梁微结构的恶化。
