Zingue Stéphane, Magne Nde Chantal Beatrice, Clyne Colin, Njamen Dieudonné
Laboratory of Physiology, Department of Life and Earth Sciences, Higher Teachers' Training College, University of Maroua, P.O. Box 55, Maroua, Cameroon; Laboratory of Animal Physiology, Department of Animal Biology and Physiology, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon.
MIMR-PHI Institute of Medical Research, Departments of Molecular and Translational Sciences, Monash University, 246 Clayton Road, Level 4, VIC 3168, Melbourne, Australia.
Int Sch Res Notices. 2014 Aug 10;2014:763781. doi: 10.1155/2014/763781. eCollection 2014.
Millettia macrophylla is used traditionally to treat menopause related symptoms. This plant was shown to exhibit estrogenic effects in vitro on human embryonic kidney cells and in vivo on ovariectomized rats. The present study aimed at elucidating underlying mechanisms by which M. macrophylla induced its estrogenic effects. To accomplish our goal, kidney Hek293T cells transiently transfected with estrogen alpha or beta receptor expression plasmids were cotreated with a pure antiestrogen ICI 182,780 and the dichloromethane or methanol soluble fractions of M. macrophylla. To follow up, we cotreated ovariectomized rats with both extracts and ICI 182,780 for 3 days in the classical uterotrophic assay. Animals were then sacrificed and the uterine wet weight, total protein levels in uteri, uterine, and vaginal epithelial heights, and mammary gland were assessed. In vitro, the results suggested that the induction of the estrogenic activity by M. macrophylla is due to the binding of its secondary metabolites to ERα and ERβ. In vivo, the cotreatment of extracts and ICI 182,780 significantly abrogated the biological responses induced by the extracts alone. Taken together, these results indicate that the active principles of M. macrophylla induce their beneficial effects on menopausal symptoms by activating the ERs.
大叶千斤拔传统上用于治疗与更年期相关的症状。这种植物在体外对人胚肾细胞以及在体内对去卵巢大鼠均显示出雌激素样作用。本研究旨在阐明大叶千斤拔产生雌激素样作用的潜在机制。为实现我们的目标，将瞬时转染雌激素α或β受体表达质粒的肾Hek293T细胞与纯抗雌激素ICI 182,780以及大叶千斤拔的二氯甲烷或甲醇可溶部分共同处理。接下来，在经典的子宫增重试验中，我们将去卵巢大鼠与这两种提取物和ICI 182,780共同处理3天。然后处死动物，评估子宫湿重、子宫中的总蛋白水平、子宫和阴道上皮高度以及乳腺。在体外，结果表明大叶千斤拔雌激素活性的诱导是由于其次级代谢产物与ERα和ERβ的结合。在体内，提取物与ICI 182,780的共同处理显著消除了单独提取物诱导的生物学反应。综上所述，这些结果表明大叶千斤拔的活性成分通过激活雌激素受体对更年期症状产生有益作用。
