Shi Lin-Jun, Zhang Chen-Yang, Zhou Zeng-Tong, Ma Jing-Yuan, Liu Yang, Bao Zhe-Xuan, Jiang Wei-Wen
Department of Oral Mucosal Diseases, Shanghai Ninth People's Hospital, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Head Neck. 2015 Jul;37(7):970-6. doi: 10.1002/hed.23700. Epub 2014 Jun 27.
Oral squamous cell carcinoma (OSCC) is a common malignancy with poor prognosis. MicroRNAs (miRNAs) play an important role in cancer, but their role in OSCC is not clarified.
We performed miRNA microarray, quantitative reverse transcription polymerase chain reaction (qRT-PCR), and fluorescence in situ hybridization (FISH) to examine miRNA expression in OSCC and paired adjacent cancer-free mucosal (ACF) tissues.
Thirteen miRNAs, including miRNA-155, were upregulated (>2-fold) in OSCC against ACF. MiRNA-155 was confirmed to have significantly higher expression in OSCC against ACF (paired-samples t test; p = .041) and it was localized in the cancer nest, inflammatory area, and vascular endothelium of OSCC. High expression of miRNA-155 in ACF tissue was an independent prognostic indicator for OSCC survival.
MiRNA-155 was overexpressed in OSCC and it was located in the cancer nest, inflammatory area, and vascular endothelium of OSCC. High miRNA-155 expression level in ACF may predict poor prognosis in patients with OSCC.
口腔鳞状细胞癌（OSCC）是一种常见的恶性肿瘤，预后较差。微小RNA（miRNA）在癌症中发挥重要作用，但其在OSCC中的作用尚不清楚。
我们进行了miRNA微阵列、定量逆转录聚合酶链反应（qRT-PCR）和荧光原位杂交（FISH），以检测OSCC及配对的相邻癌旁无瘤黏膜（ACF）组织中的miRNA表达。
与ACF相比，包括miRNA-155在内的13种miRNA在OSCC中上调（>2倍）。证实miRNA-155在OSCC中相对于ACF有显著更高的表达（配对样本t检验；p = 0.041），且其定位于OSCC的癌巢、炎症区域和血管内皮。ACF组织中miRNA-155的高表达是OSCC生存的独立预后指标。
miRNA-155在OSCC中过表达，且定位于OSCC的癌巢、炎症区域和血管内皮。ACF中miRNA-155的高表达水平可能预示OSCC患者预后不良。
