Oka Y, Nakajima K, Nagao K, Miura K, Ishii N, Kobayashi H
Department of Urology, Toho University School of Medicine , Tokyo, Japan.
J Stem Cells Regen Med. 2010 Oct 30;6(3):149-56. doi: 10.46582/jsrm.0603008. eCollection 2010.
The HEK 293 cell line (293 cells) was derived from human embryonic kidney (HEK) cells grown in tissue culture. 293 cells are very easy to grow and transfect and have been widely used in cell biological research for many years. 293 cells have many of the properties of immature neurons, suggesting that they represent a transformed neuronal cell present in the original kidney culture, and they are not useful as an in vitro model for kidney cell function. The 293T cell line contains the SV(4)0 large T-antigen, which allows the episomal replication of transfected plasmids containing the SV40 origin of replication, and 293FT cells are a fast-growing variant. A recent report showed that introducing a set of transcription factors associated with pluripotency into human somatic cells can directly reprogr am them to produce induced pluripotent stem (iPS) cells. To date, however, iPS cells have not been generated from immortalized cells. We examined whether iPS cells could be generated from 293 FT cells transfected with four transcription factors (OCT4, SOX2, NANOG, and LIN28). The obtained cells morphologically resembled human ES cells, and showed a similar marker gene expression pattern. These cells had an impaired ability to differentiate, and formed immature ectodermal tumors after they were transplanted into nude mice. Thus, we could not derive fully reprogrammed iPS cells from 293FT cells. We conclude that the 293FT cells transduced with OCT4, SOX2, NANOG, and LIN28 produced aberrant ES-like cells.
HEK 293细胞系（293细胞）源自于在组织培养中生长的人胚胎肾（HEK）细胞。293细胞非常易于生长和转染，多年来已广泛应用于细胞生物学研究。293细胞具有许多未成熟神经元的特性，这表明它们代表了原始肾培养物中存在的一种转化神经元细胞，并且它们不能作为肾细胞功能的体外模型。293T细胞系含有SV40大T抗原，这使得含有SV40复制起点的转染质粒能够进行游离复制，而293FT细胞是一种生长迅速的变体。最近的一份报告显示，将一组与多能性相关的转录因子导入人类体细胞可以直接将它们重编程以产生诱导多能干细胞（iPS细胞）。然而，迄今为止，尚未从不朽化细胞中产生iPS细胞。我们研究了用四种转录因子（OCT4、SOX2、NANOG和LIN28）转染的293FT细胞是否能够产生iPS细胞。所获得的细胞在形态上类似于人类胚胎干细胞，并显示出相似的标记基因表达模式。这些细胞的分化能力受损，并且在被移植到裸鼠体内后形成未成熟的外胚层肿瘤。因此，我们无法从293FT细胞中获得完全重编程的iPS细胞。我们得出结论，用OCT4、SOX2、NANOG和LIN28转导的293FT细胞产生了异常的胚胎干细胞样细胞。
