Zhang Ying, Cabarcas Stephanie M, Zheng J I, Sun Lei, Mathews Lesley A, Zhang Xiaohu, Lin Hongsheng, Farrar William L
Cancer Stem Cell Section, Laboratory of Cancer Prevention, National Cancer Institute-Frederick, Center for Cancer Research, Frederick, MD 21702, USA; Oncology Department, Guang An Men Hospital of China Academy of Chinese Medical Sciences, Beijing 100053, P.R. China.
Cancer Stem Cell Section, Laboratory of Cancer Prevention, National Cancer Institute-Frederick, Center for Cancer Research, Frederick, MD 21702, USA.
Oncol Lett. 2016 Jun;11(6):3803-3812. doi: 10.3892/ol.2016.4444. Epub 2016 Apr 15.
Recent evidence indicates that tumor-initiating cells (TICs), also called cancer stem cells (CSCs), are responsible for tumor initiation and progression, therefore representing an important cell population that may be used as a target for the development of future anticancer therapies. In the present study, Cryptotanshinone (CT), a traditional Chinese herbal medicine, was demonstrated to regulate the behaviors of LNCaP prostate cells and prostate LNCaP TICs. The results demonstrate that treatment with CT alters cellular proliferation, cell cycle status, migration, viability, colony formation and notably, sphere formation and down-regulation of stemness genes (Nanog, OCT4, SOX2, β-catenin, CXCR4) in TICs. The present study demonstrates that CT targets the LNCaP CD44+CD24- population that is representative of prostate TICs and also affects total LNCaP cells as well via down-regulation of stemness genes. The strong effect with which CT has on prostate TICs suggests that CT may potentially function as a novel natural anticancer agent that specifically targets TICs.
最近的证据表明，肿瘤起始细胞（TICs），也称为癌症干细胞（CSCs），负责肿瘤的起始和进展，因此代表了一个重要的细胞群体，可能被用作未来抗癌疗法开发的靶点。在本研究中，隐丹参酮（CT），一种传统的中草药，被证明可以调节LNCaP前列腺细胞和前列腺LNCaP TICs的行为。结果表明，CT处理改变了细胞增殖、细胞周期状态、迁移、活力、集落形成，特别是TICs中的球体形成和干性基因（Nanog、OCT4、SOX2、β-连环蛋白、CXCR4）的下调。本研究表明，CT靶向代表前列腺TICs的LNCaP CD44+CD24-群体，并通过下调干性基因影响总LNCaP细胞。CT对前列腺TICs的强大作用表明，CT可能潜在地作为一种新型天然抗癌剂，特异性靶向TICs。
