Liebmann L, Beetz C, Thorwarth M, Deufel T, Hübner Ca
Institute of Human Genetics , ; Institute of Clinical Chemistry .
Institute of Clinical Chemistry .
J Stem Cells Regen Med. 2012 Apr 30;8(1):35-6. doi: 10.46582/jsrm.0801006. eCollection 2012.
In vitro modelling of neuronal pathologies is, in particular, demanding and a lot of efforts have been undertaken to differentiate skin derived precursor cells into neuronal cells. However, so far all attempts did not result in cells with functional features of neurons like the ability to generate action potentials or synaptic activity. Here, we report that simple modifications of the protocols result in neuronal cells that display action potentials and synaptic activity. We think that our observation is an important step to model individual neuronal pathologies in vitro.
尤其是，神经元病理学的体外建模要求很高，并且已经付出了很多努力来将皮肤来源的前体细胞分化为神经元细胞。然而，到目前为止，所有尝试都没有产生具有神经元功能特征的细胞，如产生动作电位或突触活动的能力。在此，我们报告，对实验方案进行简单修改即可产生显示动作电位和突触活动的神经元细胞。我们认为，我们的观察结果是在体外模拟个体神经元病理学的重要一步。
