用不同去污剂解离后通过酶免疫分析检测流感病毒基质蛋白和核蛋白的抗原反应性。
Antigenic reactivity of matrix protein and nucleoprotein of influenza virus as detected by EIA after dissociation with different detergents.
作者信息
Khristova M L, Busse T L, Egorenkova E M, Leonov S V, Sokolova M V, Gitelman A K, Herrmann J, Döhner L, Kharitonenkov I G
机构信息
Ivanovsky Institute of Virology, Academy of Medical Science U.S.S.R., Moscow.
出版信息
Acta Virol. 1989 Jan;33(1):1-7.
Solid phase enzyme-immunoassay (EIA) was employed to assess the antigenic reactivity of matrix protein (M) and nucleoprotein (NP) of influenza A virus adsorbed to polystyrene in the presence of different detergents such as beta-octaglucoside (OG), Triton X-100, Tween-20, sodium dodecylsulphate (SDS), sodium deoxycholate (Doch-Na), Nonidet P-40 (NP-40), and sarcosyl at concentrations ranging from 0 to 2%. The antigenic reactivity of NP was the highest in the absence of detergents. For M protein, Doch-Na, SDS, NP-40 and sarcosyl of 0.05-0.1% enhanced the chromatophoric response in EIA 1.5-2 times. In contrast, the antigenic reactivity of M protein remained unchanged after OG or Triton X-100 treatments, and it decreased in the presence of Tween-20.
采用固相酶免疫测定法（EIA），在存在不同去污剂（如β - 八葡萄糖苷（OG）、Triton X - 100、吐温 - 20、十二烷基硫酸钠（SDS）、脱氧胆酸钠（Doch - Na）、Nonidet P - 40（NP - 40）和肌氨酸钠）的情况下，评估吸附在聚苯乙烯上的甲型流感病毒基质蛋白（M）和核蛋白（NP）的抗原反应性，去污剂浓度范围为0%至2%。在不存在去污剂的情况下，NP的抗原反应性最高。对于M蛋白，0.05 - 0.1%的Doch - Na、SDS、NP - 40和肌氨酸钠可使EIA中的发色反应增强1.5至2倍。相比之下，OG或Triton X - 100处理后M蛋白的抗原反应性保持不变，而在吐温 - 20存在的情况下其抗原反应性降低。
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