Khristova M L, Busse T L, Egorenkova E M, Leonov S V, Sokolova M V, Gitelman A K, Herrmann J, Döhner L, Kharitonenkov I G
Ivanovsky Institute of Virology, Academy of Medical Science U.S.S.R., Moscow.
Acta Virol. 1989 Jan;33(1):1-7.
Solid phase enzyme-immunoassay (EIA) was employed to assess the antigenic reactivity of matrix protein (M) and nucleoprotein (NP) of influenza A virus adsorbed to polystyrene in the presence of different detergents such as beta-octaglucoside (OG), Triton X-100, Tween-20, sodium dodecylsulphate (SDS), sodium deoxycholate (Doch-Na), Nonidet P-40 (NP-40), and sarcosyl at concentrations ranging from 0 to 2%. The antigenic reactivity of NP was the highest in the absence of detergents. For M protein, Doch-Na, SDS, NP-40 and sarcosyl of 0.05-0.1% enhanced the chromatophoric response in EIA 1.5-2 times. In contrast, the antigenic reactivity of M protein remained unchanged after OG or Triton X-100 treatments, and it decreased in the presence of Tween-20.
采用固相酶免疫测定法（EIA），在存在不同去污剂（如β - 八葡萄糖苷（OG）、Triton X - 100、吐温 - 20、十二烷基硫酸钠（SDS）、脱氧胆酸钠（Doch - Na）、Nonidet P - 40（NP - 40）和肌氨酸钠）的情况下，评估吸附在聚苯乙烯上的甲型流感病毒基质蛋白（M）和核蛋白（NP）的抗原反应性，去污剂浓度范围为0%至2%。在不存在去污剂的情况下，NP的抗原反应性最高。对于M蛋白，0.05 - 0.1%的Doch - Na、SDS、NP - 40和肌氨酸钠可使EIA中的发色反应增强1.5至2倍。相比之下，OG或Triton X - 100处理后M蛋白的抗原反应性保持不变，而在吐温 - 20存在的情况下其抗原反应性降低。
