一种在聚丙烯酰胺凝胶电泳中对过氧化物酶活性进行染色和稳定的方法。

A method for staining and stabilizing peroxidase activity in polyacrylamide gel electrophoresis.

作者信息

Shimoni M, Reuveni R

机构信息

Division of Plant Pathology, Agricultural Research Organization, Newe-Ya'ar, Israel.

出版信息

Anal Biochem. 1988 Nov 15;175(1):35-8. doi: 10.1016/0003-2697(88)90357-0.

DOI:10.1016/0003-2697(88)90357-0

PMID:2469356

Abstract

A procedure was developed for a rapid double staining of peroxidase and other proteins in the same polyacrylamide gels using guaiacol and Coomassie blue. The distinguishable colored bands of peroxidase isozymes and proteins are stable for at least 8 months.

摘要

开发了一种使用愈创木酚和考马斯亮蓝在同一聚丙烯酰胺凝胶中对过氧化物酶和其他蛋白质进行快速双重染色的方法。过氧化物酶同工酶和蛋白质的可区分色带至少稳定8个月。