Fieldes M A, Starobin J, Tyson H
Anal Biochem. 1984 Feb;137(1):146-50. doi: 10.1016/0003-2697(84)90361-0.
The staining of both peroxidases (PERs) and acid phosphatases (APs) following electrophoresis on acrylamide gels is described. Scanning doubly stained gels at appropriate wavelengths reveals individual isozyme bands for both enzyme systems, giving essentially identical results to those obtained from separately run and stained gels. Collection of relative mobility (Rm) data is unaffected by such tandem staining. Few complications arise from overlapping PER and AP bands, or interactions between the two staining methods and PER or AP isozymes. Even if repeat scans of a gel at different wavelengths are needed to retrieve all isozyme information, dual staining provides distinct savings in time and sample material.
本文描述了在丙烯酰胺凝胶上进行电泳后对过氧化物酶（PERs）和酸性磷酸酶（APs）的染色方法。在适当波长下扫描双重染色的凝胶，可显示两种酶系统的各个同工酶条带，其结果与分别进行电泳和染色的凝胶所得结果基本相同。相对迁移率（Rm）数据的收集不受这种串联染色的影响。PER和AP条带重叠，或两种染色方法与PER或AP同工酶之间的相互作用很少产生并发症。即使需要在不同波长下对凝胶进行重复扫描以获取所有同工酶信息，双重染色也能显著节省时间和样品材料。
