School of Material and Chemical Engineering, Bengbu University, Bengbu 233030, P. R. China.
Analyst. 2019 Mar 11;144(6):1975-1981. doi: 10.1039/c8an02192a.
In this article, we proposed new nitrogen-doped boronic acid-decorated carbon nanodots (CNDs) for the recognition and detection of glycoproteins. These doped, decorated CNDs were obtained by a one-step hydrothermal carbonization method using phenylboronic acid and ethylenediamine as precursors. Compared to traditional synthesized and then functionalized nanoscale sensing systems, this method is more facile and efficient. The as-prepared nitrogen-doped CNDs possessed a quasi-spherical morphology and a high quantum yield of approximately 14.5%. The added glycoproteins (taking horseradish peroxidase as a model protein) can selectively induce the assembly and fluorescence quenching of CNDs through the formation of cyclic boronate esters, because the boronic acid groups on the CND surfaces can covalently interact with cis-diol-containing glycoproteins. These fluorescence responses can be used to properly quantify horseradish peroxidase in the range of 3.3-333.3 μg mL-1 with a detection limit of 0.52 μg mL-1, and the selectivity assay with functionalized CNDs was further investigated using various proteins with different quantities of glycosylation sites as well as using smaller molecules. The results show that the nanosensing system possesses favorable selectivity. Due to its simplicity and effectiveness, the system has great application prospects as a practical platform for glycoprotein sensing.
在本文中，我们提出了一种新的氮掺杂硼酸修饰的碳点（CND）用于糖蛋白的识别和检测。这些掺杂、修饰的 CND 通过一步水热碳化法，以苯硼酸和乙二胺为前体制备得到。与传统的合成后功能化的纳米级传感系统相比，这种方法更加简便高效。所制备的氮掺杂 CND 具有准球形形态和约 14.5%的高量子产率。加入的糖蛋白（以辣根过氧化物酶为模型蛋白）可以通过形成环状硼酸酯选择性地诱导 CND 的组装和荧光猝灭，因为 CND 表面上的硼酸基团可以与含有顺式二羟基的糖蛋白发生共价相互作用。这些荧光响应可用于在 3.3-333.3μgmL-1范围内适当定量辣根过氧化物酶，检测限为 0.52μgmL-1，并且使用具有不同糖基化位点数量的各种蛋白质以及使用较小的分子进一步研究了功能化 CND 的选择性测定。结果表明，该纳米传感系统具有良好的选择性。由于其简单有效，该系统具有作为糖蛋白传感实用平台的巨大应用前景。
