Poidevin Laetitia, Berrin Jean-Guy, Bennati-Granier Chloé, Levasseur Anthony, Herpoël-Gimbert Isabelle, Chevret Didier, Coutinho Pedro M, Henrissat Bernard, Heiss-Blanquet Senta, Record Eric
Biotechnologie des Champignons Filamenteux, BCF, UMR 1163, INRA, Aix Marseille Université, Polytech, CP 925, 13228, Marseille Cedex 09, France.
Appl Microbiol Biotechnol. 2014 Sep;98(17):7457-69. doi: 10.1007/s00253-014-5698-3. Epub 2014 Apr 3.
The genome of the coprophilous fungus Podospora anserina harbors a large and highly diverse set of putative lignocellulose-acting enzymes. In this study, we investigated the enzymatic diversity of a broad range of P. anserina secretomes induced by various carbon sources (dextrin, glucose, xylose, arabinose, lactose, cellobiose, saccharose, Avicel, Solka-floc, birchwood xylan, wheat straw, maize bran, and sugar beet pulp (SBP)). Compared with the Trichoderma reesei enzymatic cocktail, P. anserina secretomes displayed similar cellulase, xylanase, and pectinase activities and greater arabinofuranosidase, arabinanase, and galactanase activities. The secretomes were further tested for their capacity to supplement a T. reesei cocktail. Four of them improved significantly the saccharification yield of steam-exploded wheat straw up to 48 %. Fine analysis of the P. anserina secretomes produced with Avicel and SBP using proteomics revealed a large array of CAZymes with a high number of GH6 and GH7 cellulases, CE1 esterases, GH43 arabinofuranosidases, and AA1 laccase-like multicopper oxidases. Moreover, a preponderance of AA9 (formerly GH61) was exclusively produced in the SBP condition. This study brings additional insights into the P. anserina enzymatic machinery and will facilitate the selection of promising targets for the development of future biorefineries.
粪生真菌嗜鸟粪拟青霉的基因组含有大量高度多样的假定木质纤维素作用酶。在本研究中，我们调查了由各种碳源（糊精、葡萄糖、木糖、阿拉伯糖、乳糖、纤维二糖、蔗糖、微晶纤维素、脱脂棉、桦木木聚糖、小麦秸秆、玉米麸皮和甜菜浆（SBP））诱导产生的多种嗜鸟粪拟青霉分泌蛋白组的酶多样性。与里氏木霉的酶混合物相比，嗜鸟粪拟青霉分泌蛋白组表现出相似的纤维素酶、木聚糖酶和果胶酶活性，以及更高的阿拉伯呋喃糖苷酶、阿拉伯聚糖酶和半乳聚糖酶活性。进一步测试了这些分泌蛋白组补充里氏木霉混合物的能力。其中四种显著提高了蒸汽爆破小麦秸秆的糖化产率，最高可达48%。使用蛋白质组学对由微晶纤维素和甜菜浆产生的嗜鸟粪拟青霉分泌蛋白组进行精细分析，发现了大量的碳水化合物活性酶，其中包括大量的GH6和GH7纤维素酶、CE1酯酶、GH43阿拉伯呋喃糖苷酶和AA1漆酶样多铜氧化酶。此外，大量的AA9（以前的GH61）仅在甜菜浆条件下产生。本研究为嗜鸟粪拟青霉的酶机制带来了更多见解，并将有助于为未来生物炼制厂的发展选择有前景的目标。
