Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands; Department of Food and Environmental Sciences, Division of Microbiology and Biotechnology, P.O. Box 56, Viikinkaari 9, University of Helsinki, Helsinki, Finland, Finland.
Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands; Microbiology, Utrecht University, Padualaan 8, 3584 Ch Utrecht, The Netherlands.
N Biotechnol. 2017 Jul 25;37(Pt B):162-171. doi: 10.1016/j.nbt.2017.02.002. Epub 2017 Feb 7.
The coprophilic ascomycete fungus Podospora anserina was cultivated on three different plant biomasses, i.e. cotton seed hulls (CSH), soybean hulls (SBH) and acid-pretreated wheat straw (WS) for four days, and the potential of the produced enzyme mixtures was compared in the enzymatic saccharification of the corresponding lignocellulose feedstocks. The enzyme cocktail P. anserina produced after three days of growth on SBH showed superior capacity to release reducing sugars from all tested plant biomass feedstocks compared to the enzyme mixtures from CSH and WS cultures. Detailed proteomics analysis of the culture supernatants revealed that SBH contained the most diverse set of enzymes targeted on plant cell wall polymers and was particularly abundant in xylan, mannan and pectin acting enzymes. The importance of lytic polysaccharide monooxygenases (LPMOs) in plant biomass deconstruction was supported by identification of 20 out of 33 AA9 LPMOs in the SBH cultures. The results highlight the suitability of P. anserina as a source of plant cell wall degrading enzymes for biotechnological applications and the importance of selecting the most optimal substrate for the production of enzyme mixtures.
腐生性子囊菌 Podospora anserina 在三种不同的植物生物质上培养了四天，即棉籽壳 (CSH)、大豆壳 (SBH) 和酸预处理的小麦秸秆 (WS)，并比较了相应木质纤维素饲料酶解中产生的酶混合物的潜力。在 SBH 上生长三天后产生的 Podospora anserina 酶混合物与 CSH 和 WS 培养物的酶混合物相比，具有从所有测试的植物生物质饲料中释放还原糖的更高能力。对培养上清液的详细蛋白质组学分析表明，SBH 含有针对植物细胞壁聚合物的最多样化的酶组，并且特别富含木聚糖、甘露聚糖和果胶作用酶。溶菌多糖单加氧酶 (LPMOs) 在植物生物质解构中的重要性得到了支持，因为在 SBH 培养物中鉴定出了 33 个 AA9 LPMOs 中的 20 个。这些结果突出了 Podospora anserina 作为用于生物技术应用的植物细胞壁降解酶的来源的适宜性，以及选择最优化的底物来生产酶混合物的重要性。
