Tan Kah Hin, Ki Kitti Chan Wing, Watanabe Satoru, Vasudevan Subhash G, Krishnan Manoj
Emerging Infectious Diseases Program, Duke-NUS Graduate Medical School, Singapore, Singapore.
Methods Mol Biol. 2014;1138:99-109. doi: 10.1007/978-1-4939-0348-1_7.
Large-scale screening of antiviral compounds that target dengue virus life cycle requires a robust cell-based assay that is rapid, easy to conduct, and sensitive enough to be able to assess viral infectivity and cell viability so that antiviral efficacy can be measured. In this chapter we describe a method that uses high-content imaging to evaluate the in vitro antiviral efficacy in a modification to the cell-based flavivirus immunodetection (CFI) assay that was described previously in Wang et al. (Antimicrob Agents Chemother 53(5):1823-1831, 2009).
大规模筛选针对登革热病毒生命周期的抗病毒化合物需要一种强大的基于细胞的检测方法，该方法要快速、易于操作且足够灵敏，能够评估病毒感染性和细胞活力，以便测量抗病毒效果。在本章中，我们描述了一种方法，该方法使用高内涵成像来评估体外抗病毒效果，它是对Wang等人（《抗菌药物与化疗》53(5):1823 - 1831, 2009）之前描述的基于细胞的黄病毒免疫检测（CFI）方法的一种改进。
