Mangalgi Smita, Sajjan Annapurna
Department of Microbiology, BLDEU's Shri B. M. Patil Medical College, Bijapur, Karnataka, India.
J Lab Physicians. 2014 Jan;6(1):14-7. doi: 10.4103/0974-2727.129084.
Isolation of Brucella is the gold standard in the laboratory diagnosis of brucellosis. As Brucella is intracellular and the number of circulating bacteria is usually low, removal/dilution of antibacterial substances, concentration of bacteria and optimal culture conditions may enhance the rate of isolation.
The objective of the following study was to compare the lysis concentration (LC), clot culture and conventional Castaneda blood culture techniques for the isolation rate and recovery time in the diagnosis of human brucellosis.
Blood cultures by LC, clot culture and conventional method were performed in 169 patients who had antibody titers ≥160 international units by the serum agglutination test.
Overall blood culture positivity was found to be 24.8%, 43.1% and 34.9% by conventional, LC and clot culture techniques in that order. The mean recovery time by LC and clot culture techniques was significantly less than conventional method, resulting in an overall difference of nearly 6 and 4 days respectively.
For the isolation of Brucella from blood, LC and clot culture techniques are better than the conventional technique.
布鲁氏菌的分离是布鲁氏菌病实验室诊断的金标准。由于布鲁氏菌是细胞内寄生菌且循环细菌数量通常较低，去除/稀释抗菌物质、浓缩细菌以及优化培养条件可能会提高分离率。
以下研究的目的是比较裂解浓度（LC）、凝块培养和传统的卡斯塔涅达血培养技术在人类布鲁氏菌病诊断中的分离率和恢复时间。
对169例血清凝集试验抗体滴度≥160国际单位的患者进行了LC、凝块培养和传统方法的血培养。
传统、LC和凝块培养技术的总体血培养阳性率依次为24.8%、43.1%和34.9%。LC和凝块培养技术的平均恢复时间明显短于传统方法，总体差异分别约为6天和4天。
对于从血液中分离布鲁氏菌，LC和凝块培养技术优于传统技术。
