在布鲁氏菌病流行地区对七种用于诊断人类布鲁氏菌病的检测方法进行评估。
Evaluation of seven tests for diagnosis of human brucellosis in an area where the disease is endemic.
作者信息
Gómez M Concepción, Nieto José A, Rosa Carmen, Geijo Paloma, Escribano M Angeles, Muñoz Amparo, López Clara
机构信息
Microbiology Laboratory Hospital Severo Ochoa (Leganés), Madrid, Spain.
出版信息
Clin Vaccine Immunol. 2008 Jun;15(6):1031-3. doi: 10.1128/CVI.00424-07. Epub 2008 Apr 30.
Abstract
The results of seven serologic tests for diagnosis of human brucellosis were evaluated. The titrated Rose Bengal test, microagglutination test, microtiter-adapted Coombs test, and immunocapture-agglutination test (Brucellacapt) were positive for all sera from patients with acute brucellosis. The immunoglobulin G (IgG), IgM, and IgA commercial enzyme immunoassays (ELISAs) failed to show specific antibodies in 3 patients, 10 patients, and 1 patient, respectively. The sensitivity of ELISA is not higher than that of conventional tests.
摘要
对用于诊断人类布鲁氏菌病的七种血清学检测结果进行了评估。滴定玫瑰红试验、微量凝集试验、微量滴定改良库姆斯试验和免疫捕获凝集试验（Brucellacapt）对所有急性布鲁氏菌病患者的血清均呈阳性。免疫球蛋白G（IgG）、IgM和IgA商业酶免疫测定法（ELISA）分别在3例、10例和1例患者中未显示出特异性抗体。ELISA的敏感性不高于传统检测方法。
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