Hasanein Parisa, Ghafari-Vahed Masumeh, Khodadadi Iraj
a Department of Biology , School of Basic Sciences, Bu-Ali Sina University , Hamedan , Iran.
b Department of Medical Biochemistry , School of Medicine, Hamedan University of Medical Sciences , Iran.
Redox Rep. 2017 Jan;22(1):42-50. doi: 10.1080/13510002.2016.1140406. Epub 2016 Feb 15.
Liver is considered a target organ affected by lead toxicity. Oxidative stress is among the mechanisms involved in liver damage. Here we investigated the effects of the natural alkaloid berberine on oxidative stress and hepatotoxicity induced by lead in rats.
Animals received an aqueous solution of lead acetate (500 mg Pb/l in the drinking water) and/or daily oral gavage of berberine (50 mg/kg) for 8 weeks. Rats were then weighed and used for the biochemical, molecular, and histological evaluations.
Lead-induced oxidative stress, shown by increasing lipid peroxidation along with a concomitant decrease in hepatic levels of thiol groups, total antioxidant capacity, the activities of superoxide dismutase, catalase, glutathione peroxidase, and glutathione-S-transferase, and reduced versus oxidized glutathione ratio. Berberine corrected all the disturbances in oxidative stress markers induced by lead administration. Berberine also prevented the elevated levels of enzymes (alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase) and the decrease in body weight and albumin. The protective effects of berberine were comparable with silymarin. Furthermore, berberine attenuated liver damage, shown by decreased necrosis and inflammatory cell infiltration.
Berberine represents a potential therapeutic option against lead-induced hepatotoxicity through inhibiting lipid peroxidation and enhancing antioxidant defenses.
Berberine exerted protective effects on lead-induced oxidative stress and hepatotoxicity in rats.
肝脏被认为是受铅毒性影响的靶器官。氧化应激是肝脏损伤所涉及的机制之一。在此，我们研究了天然生物碱黄连素对铅诱导的大鼠氧化应激和肝毒性的影响。
动物饮用含醋酸铅的水溶液（饮用水中500毫克铅/升）和/或每日口服黄连素（50毫克/千克），持续8周。然后对大鼠称重，并用于生化、分子和组织学评估。
铅诱导氧化应激，表现为脂质过氧化增加，同时肝脏中巯基水平、总抗氧化能力、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽-S-转移酶的活性降低，以及还原型谷胱甘肽与氧化型谷胱甘肽的比例降低。黄连素纠正了铅给药诱导的氧化应激标志物的所有紊乱。黄连素还预防了酶（丙氨酸转氨酶、天冬氨酸转氨酶和碱性磷酸酶）水平的升高以及体重和白蛋白的降低。黄连素的保护作用与水飞蓟宾相当。此外，黄连素减轻了肝脏损伤，表现为坏死和炎性细胞浸润减少。
黄连素通过抑制脂质过氧化和增强抗氧化防御，代表了一种对抗铅诱导的肝毒性的潜在治疗选择。
黄连素对铅诱导的大鼠氧化应激和肝毒性发挥了保护作用。
