Cornette J C, Sharma S K
Upjohn Company, Kalamazoo, MI 49001.
J Immunoassay. 1989;10(1):107-24. doi: 10.1080/01971528908053230.
We report the preparation and application of polyclonal antisera to the analysis and quantitation of human interleukin-1 alpha (IL-1 alpha) and human interleukin-1 beta (IL-1 beta). The anti IL-1 alpha antibodies specifically react with the alpha form of IL-1 and do not cross react (less than 0.1%) with the beta form of IL-1 and vice versa. Data reported here demonstrate that detection of human IL-1 alpha or beta by a radioimmunoassay technique is sensitive enough to measure picogram levels of these lymphokines. The practical application of using these highly specific antisera for radioimmunoassays was established by measuring exogenously added IL-1 alpha or IL-1 beta to human plasma. Potential benefits of these reagents and the radioimmunoassay procedures described herein are discussed in relation to the biological assays which cannot distinguish between human IL-1 alpha and human IL-1 beta.
我们报告了多克隆抗血清的制备及其在人白细胞介素 -1α（IL -1α）和人白细胞介素 -1β（IL -1β）分析及定量中的应用。抗IL -1α抗体与IL -1的α形式特异性反应，与IL -1的β形式无交叉反应（小于0.1%），反之亦然。此处报告的数据表明，通过放射免疫测定技术检测人IL -1α或β的灵敏度足以测量这些淋巴因子的皮克水平。通过测量添加到人体血浆中的外源性IL -1α或IL -1β，确立了使用这些高度特异性抗血清进行放射免疫测定的实际应用。本文所述试剂和放射免疫测定程序的潜在益处与无法区分人IL -1α和人IL -1β的生物学测定相关进行了讨论。
