Bristow A F, Rafferty B, Selkirk S, Poole S
National Institute for Biological Standards and Control, Potters Bar, Herts, U.K.
Dev Biol Stand. 1988;69:103-9.
Polyclonal anti-interleukin-1 beta (IL-1 beta) sera raised in sheep and in rabbits bound to unlabelled IL-1 beta. IL-1 beta radioiodinated using chloramine-T or Bolton-Hunter procedures was bound by the anti-IL-1 beta sera but was not displaced by unlabelled IL-1 beta suggesting that IL-1 beta was damaged during iodination procedures, resulting in alterations to the immunological properties of the tracer. Iodination using the mild oxidant N-bromosuccinimide, followed by extensive tracer purification to remove the unlabelled IL-1 beta, produced a tracer with a specific activity of 89 microCi/micrograms (0.83 atoms iodine/molecule IL-1) which was fully displaced by unlabelled IL-1 beta, allowing the development of a sensitive and specific radioimmunoassay for IL-1 beta. The radioimmunoassay has been used to quantify intracellular IL-1 beta and IL-1 beta release by peripheral blood monocytes stimulated with endotoxin.
在绵羊和兔子体内产生的抗白细胞介素 -1β（IL-1β）多克隆血清与未标记的IL-1β结合。使用氯胺 -T或博尔顿 -亨特法进行放射性碘化的IL-1β能被抗IL-1β血清结合，但未被未标记的IL-1β取代，这表明IL-1β在碘化过程中受到了损伤，导致示踪剂的免疫特性发生改变。使用温和氧化剂N-溴代琥珀酰亚胺进行碘化，随后对示踪剂进行广泛纯化以去除未标记的IL-1β，得到了一种比活度为89微居里/微克（0.83个碘原子/分子IL-1）的示踪剂，它能被未标记的IL-1β完全取代，从而得以开发出一种灵敏且特异的IL-1β放射免疫测定法。该放射免疫测定法已用于对内毒素刺激的外周血单核细胞释放的细胞内IL-1β和IL-1β进行定量。
