Chieochansin Thaweesak, Vutithanachot Viboonsak, Theamboonlers Apiradee, Poovorawan Yong
Clin Lab. 2014;60(3):511-4. doi: 10.7754/clin.lab.2013.130616.
Rapid tests are widely used to detect rotavirus A; however, pediatricians are concerned whether the rapid test can still accurately detect the virus. Therefore, this study evaluated the performance of the rotavirus rapid test by comparing it to the one-step RT-PCR method.
Seven hundred fifty-five stool samples were collected from children with acute diarrhea. All samples were processed immediately after arrival with the SD BIOLINE rota rapid test and one-step RT-PCR method.
The detection rates of rotavirus A were 40.79% and 41.91% for the rapid test and one-step RT-PCR, respectively. The rapid test had 93.57% sensitivity and 96.17% specificity. Most of the different genotypes of rotavirus A were detected with the SD rapid test.
Although the rapid test is able to quickly give results, we found that it has high false positive and negative rates. Thus, other highly sensitive methods such as one-step RT-PCR are still required for true diagnosis.
快速检测广泛用于检测A组轮状病毒；然而，儿科医生担心快速检测是否仍能准确检测出该病毒。因此，本研究通过将轮状病毒快速检测与一步法逆转录聚合酶链反应（RT-PCR）方法进行比较，评估了该快速检测的性能。
从急性腹泻儿童中收集了755份粪便样本。所有样本在送达后立即用SD BIOLINE轮状病毒快速检测和一步法RT-PCR方法进行处理。
快速检测和一步法RT-PCR检测A组轮状病毒的检出率分别为40.79%和41.91%。快速检测的灵敏度为93.57%，特异性为96.17%。使用SD快速检测可检测出大多数不同基因型的A组轮状病毒。
尽管快速检测能够快速给出结果，但我们发现其假阳性和假阴性率较高。因此，真正的诊断仍需要其他高灵敏度方法，如一步法RT-PCR。
