Husain M, Seth P, Broor S
Department of Microbiology, All India Institute of Medical Sciences, New Delhi.
Arch Virol. 1995;140(7):1225-33. doi: 10.1007/BF01322748.
RT-PCR was employed to detect rotavirus infection in 450 fecal samples from children with acute diarrhoea. It was compared with enzyme-linked immunosorbent assay (ELISA) and polyacrylamide gel electrophoresis (PAGE) for rotavirus detection. A total of 67 samples were found positive by at least one of the three techniques. Of these 67 samples, 51 were positive by all three methods, 6 were positive by ELISA and RT-PCR but negative by PAGE, 3 were positive by PAGE and RT-PCR and negative by ELISA, 3 and 4 samples were exclusively positive by RT-PCR and ELISA respectively. These results indicate that RT-PCR is a sensitive and specific assay for detection of group A rotaviruses in stool samples from cases of acute diarrhoea.
采用逆转录聚合酶链反应（RT-PCR）检测450例急性腹泻儿童粪便样本中的轮状病毒感染情况。将其与酶联免疫吸附测定（ELISA）和聚丙烯酰胺凝胶电泳（PAGE）检测轮状病毒的结果进行比较。通过这三种技术中的至少一种检测发现，共有67份样本呈阳性。在这67份样本中，51份通过所有三种方法检测均为阳性，6份通过ELISA和RT-PCR检测为阳性，但PAGE检测为阴性，3份通过PAGE和RT-PCR检测为阳性，ELISA检测为阴性，分别有3份和4份样本仅通过RT-PCR和ELISA检测呈阳性。这些结果表明，RT-PCR是检测急性腹泻病例粪便样本中A组轮状病毒的一种灵敏且特异的检测方法。
