Shakya Garima, Manjini Selva, Hoda Muddasarul, Rajagopalan Rukkumani
J Basic Clin Physiol Pharmacol. 2014 Feb;25(1):73-9. doi: 10.1515/jbcpp-2013-0051.
The present study aimed to analyze the effect of kaempferol on oxidative stress induced by alcohol and thermally oxidized polyunsaturated fatty acid (ΔPUFA) in male albino Wistar rats.
The rats were divided into four groups. The animals in group 1 served as the normal group (standard diet), group 2 served as the hepatotoxic group (alcohol+ΔPUFA), group 3 served as the treated group (alcohol+ΔPUFA+kaempferol), and group 4 served as kaempferol control. The levels of marker enzyme γ-glutamyl transferase (GGT), lipid peroxidation markers [thiobarbituric acid reactive substances (TBARS) and lipid hydroperoxides (LH)], enzymatic antioxidants (catalase, superoxide dismutase, and glutathione peroxidase), and nonenzymatic antioxidants (reduced glutathione, vitamin E, and vitamin C) were analyzed in liver to evaluate the effects of kaempferol.
The levels of GGT, TBARS, and LH were significantly increased in liver of the alcohol+ΔPUFA group and were found to be reduced on treatment with kaempferol. The levels of both enzymatic and nonenzymatic antioxidants were decreased in liver of the alcohol+ΔPUFA group and were found to be restored on treatment with kaempferol.
From the results obtained, we conclude that kaempferol protects the liver against alcohol- and ΔPUFA- induced oxidative stress.
本研究旨在分析山奈酚对雄性白化Wistar大鼠因酒精和热氧化多不饱和脂肪酸（ΔPUFA）诱导的氧化应激的影响。
将大鼠分为四组。第1组动物作为正常组（标准饮食），第2组作为肝毒性组（酒精+ΔPUFA），第3组作为治疗组（酒精+ΔPUFA+山奈酚），第4组作为山奈酚对照组。分析肝脏中标记酶γ-谷氨酰转移酶（GGT）、脂质过氧化标记物[硫代巴比妥酸反应性物质（TBARS）和脂质氢过氧化物（LH）]、酶促抗氧化剂（过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶）以及非酶促抗氧化剂（还原型谷胱甘肽、维生素E和维生素C）的水平，以评估山奈酚的作用。
酒精+ΔPUFA组肝脏中GGT、TBARS和LH的水平显著升高，而用山奈酚治疗后这些水平降低。酒精+ΔPUFA组肝脏中酶促和非酶促抗氧化剂的水平均降低，而用山奈酚治疗后这些水平得以恢复。
根据获得的结果，我们得出结论，山奈酚可保护肝脏免受酒精和ΔPUFA诱导的氧化应激。
