Tasker S, Barker E N, Day M J, Helps C R
J Small Anim Pract. 2014 Apr;55(4):185-9. doi: 10.1111/jsap.12180.
The aim of this study was to determine the agreement between AB blood phenotyping and genotyping and determine whether non-AB blood type incompatibilities exist in UK cats.
Blood samples underwent phenotyping (A, B or AB) using microplate agglutination, and genotyping (AA, Ab or bb) using pyrosequencing of a fragment of the cytidine monophospho-N-acetylneuraminic acid hydroxylase gene. Non-AB blood type incompatibilities were investigated by cross-matching against reference blood of the same phenotype.
Of 112 cats tested, 86 (77%) were blood phenotype A, 19 (17%) type B and 7 (6%) type AB. Genotype and initial phenotype agreed in 96% (107 of 112) of cats, but 5 were discordant; these were all B phenotype with either AA (n=2) or Ab (n=3) genotype. Two of the five cats had repeat blood samples tested: one was reclassified as phenotype A; the other remained phenotype B. Two cats had incompatibilities on minor cross-match, but these were attributed to phenotyping errors.
Unknown mutation(s) associated with phenotype B, resulting in false AA or Ab genotyping, were evident in a small number of cases in this study. No conclusive evidence for non-AB blood type incompatibilities was found.
本研究旨在确定AB血型表型与基因分型之间的一致性，并确定英国猫中是否存在非AB血型不相容性。
血样通过微孔板凝集进行血型表型鉴定（A、B或AB型），并使用胞苷单磷酸-N-乙酰神经氨酸羟化酶基因片段的焦磷酸测序进行基因分型（AA、Ab或bb型）。通过与相同表型的参考血液进行交叉配血来研究非AB血型不相容性。
在112只受试猫中，86只（77%）为A型血，19只（17%）为B型血，7只（6%）为AB型血。96%（112只中的107只）的猫基因型与初始表型一致，但有5只不一致；这些均为B型表型，基因型为AA（n = 2）或Ab（n = 3）。五只猫中的两只再次进行了血样检测：一只重新分类为A型血；另一只仍为B型血。两只猫在次要交叉配血时有不相容情况，但这些归因于血型表型鉴定错误。
本研究中少数病例存在与B型表型相关的未知突变，导致AA或Ab基因分型错误。未发现非AB血型不相容的确切证据。
