Uno Yumiko, Yaguchi Masami, Kobayashi Tasuku, Onozawa Eri, Ochiai Kazuhiko, Yoshida Karin, Nakamura Chihiro, Udagawa Chihiro, Omi Toshinori
Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
Anifa Animal Hospital Kashiwa, Chiba, Japan.
Front Vet Sci. 2021 Sep 13;8:720445. doi: 10.3389/fvets.2021.720445. eCollection 2021.
The feline AB blood group system (blood types A, B, and AB) encoding the cytidine monophosphate-N-acetylneuraminic acid hydroxylase () gene is the most significant in transfusion medicine and hemolysis of the newborn for cats. Blood typing and cross-matching in pre-transfusion testing are crucial to determining blood compatibility and thus prevent hemolytic transfusion reactions. We here performed serological and genetic investigations to characterize blood samples from cats with discordant results for card agglutination (CARD) and the alloantibody agglutination test for blood typing in two cats (subjects K and R). Subject K showed incompatible cross-matching in pre-transfusion testing. Red blood cells from subjects K and R determined blood type B from the CARD method showed blood type AB by alloanti-A and alloanti-B antibodies in agglutination testing. Genomic DNA sequencing of the coding region (exons 1a to 14) for the cat gene showed that subject K had four mutations with heterozygosity at c.139C>T, c.179G>T, c.327A>C, and c.364C>T. Similarly, the gene of subject R carried six mutations with heterozygosity at c.142G>A, c.187A>G, c.268T>A, c.327A>C, c.773G>A and c.1603G>A, representing a new diplotype including a novel synonymous single nucleotide polymorphism (SNP) in exon 7 (c.773 G>A: Arg258Gln). The diplotype in subjects K and R was different from major diplotype in blood type B cats. This study is the first to report variants in cats with discordant blood types between CARD and TUBE methods. These results could assist in the classification of feline AB blood types for transfusion medicine to avoid blood incompatibilities.
猫AB血型系统（血型A、B和AB）由胞苷单磷酸-N-乙酰神经氨酸羟化酶（）基因编码，在猫的输血医学和新生儿溶血中最为重要。输血前检测中的血型鉴定和交叉配血对于确定血液相容性从而预防溶血性输血反应至关重要。我们在此进行了血清学和遗传学研究，以对两只猫（K和R个体）的卡片凝集（CARD）和血型鉴定的同种抗体凝集试验结果不一致的猫的血样进行特征分析。K个体在输血前检测中显示交叉配血不相容。通过CARD方法确定为B型血的K和R个体的红细胞，在凝集试验中通过抗A和抗B同种抗体显示为AB型血。猫基因编码区（外显子1a至14）的基因组DNA测序表明，K个体在c.139C>T、c.179G>T、c.327A>C和c.364C>T处有四个杂合突变。同样，R个体的基因有六个杂合突变，分别位于c.142G>A、c.187A>G、c.268T>A、c.327A>C、c.773G>A和c.1603G>A，代表一种新的双倍型，包括外显子7中的一个新的同义单核苷酸多态性（SNP）（c.773 G>A：Arg258Gln）。K和R个体的双倍型与B型血猫的主要双倍型不同。本研究首次报道了CARD和试管法血型不一致的猫中的变体。这些结果有助于输血医学中猫AB血型的分类，以避免血液不相容。
