Chen Lin, DiPietro Luisa A
Center for Wound Healing and Tissue Regeneration, University of Illinois at Chicago, Chicago, IL, USA.
Exp Dermatol. 2014 Jun;23(6):436-8. doi: 10.1111/exd.12411.
Pigment epithelium-derived factor (PEDF) is a multifunctional factor with potent anti-angiogenic activity that may play a role in skin homoeostasis and wound healing. Analysis of PEDF levels demonstrated that PEDF levels are high in normal skin but quite low in early wounds. As previous studies have suggested that keratinocytes can produce PEDF, we investigated how conditions that mimic those found at sites of injury influence PEDF production by keratinocytes in vitro. Both injury by mechanical disruption (scratch assay) and treatment of human keratinocytes with inflammatory cytokines (IL-1β, IL-6 and TNF-α) inhibited PEDF expression. We next examined how PEDF affects keratinocyte functions that are important in tissue repair. Treatment of keratinocytes with exogenous PEDF enhanced keratinocyte adhesion, therefore impairing migration, while having no effect on cell proliferation. The results suggest that modulation of PEDF levels may play a pivotal role in skin homoeostasis and the response of keratinocytes to injury or inflammatory insults.
色素上皮衍生因子（PEDF）是一种具有强大抗血管生成活性的多功能因子，可能在皮肤稳态和伤口愈合中发挥作用。对PEDF水平的分析表明，PEDF在正常皮肤中水平较高，但在早期伤口中相当低。正如先前的研究表明角质形成细胞可以产生PEDF，我们研究了模拟损伤部位条件的情况如何在体外影响角质形成细胞产生PEDF。机械破坏损伤（划痕试验）和用炎性细胞因子（IL-1β、IL-6和TNF-α）处理人角质形成细胞均抑制PEDF表达。接下来，我们研究了PEDF如何影响组织修复中重要的角质形成细胞功能。用外源性PEDF处理角质形成细胞增强了角质形成细胞的黏附，从而损害了迁移，而对细胞增殖没有影响。结果表明，PEDF水平的调节可能在皮肤稳态以及角质形成细胞对损伤或炎性刺激的反应中起关键作用。
