大肠杆菌O36 O抗原的结构与基因簇
Structure and gene cluster of the O-antigen of Escherichia coli O36.
作者信息
Perepelov Andrei V, Wang Quan, Levina Evgeniya A, Ovchinnikova Olga G, Qian Ye, Shashkov Alexander S, Wang Lei, Knirel Yuriy A
机构信息
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
出版信息
Carbohydr Res. 2014 May 22;390:46-9. doi: 10.1016/j.carres.2014.01.008. Epub 2014 Jan 14.
The O-polysaccharide (O-antigen) of Escherichia coli O36 was isolated from the lipopolysaccharide and studied by sugar analyses and Smith degradation along with (1)H and (13)C NMR spectroscopy. The following structure of the branched pentasaccharide repeating unit was established, which is unique among the known structures of bacterial polysaccharides: The O-antigen gene cluster of E. coli O36 has been sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the O-polysaccharide structure.
从大肠杆菌O36的脂多糖中分离出O-多糖（O-抗原），并通过糖分析、史密斯降解以及¹H和¹³C核磁共振光谱进行研究。确定了以下分支五糖重复单元的结构，这在已知的细菌多糖结构中是独一无二的：已对大肠杆菌O36的O-抗原基因簇进行了测序。通过与现有数据库中的序列进行比较，初步确定了基因功能，发现与O-多糖结构完全一致。
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