N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Carbohydr Res. 2012 Nov 1;361:73-7. doi: 10.1016/j.carres.2012.07.024. Epub 2012 Aug 14.
The O-polysaccharide (O-antigen) of Escherichia coli O102 was studied by sugar analysis along with one- and two-dimensional (1)H and (13)C NMR spectroscopy. The following structure of the branched pentasaccharide repeating unit was established: [formula: see text]. The O-antigen gene cluster of E. coli O102 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the E. coli O102 O-polysaccharide structure.
采用糖分析和一维及二维(1)H 和(13)C NMR 光谱法研究了大肠杆菌 O102 的 O-多糖(O-抗原)。确定了支化五糖重复单元的以下结构:[化学式:见正文]。测序了大肠杆菌 O102 的 O-抗原基因簇。通过与现有数据库中的序列进行比较,推测了基因的功能,并与大肠杆菌 O102 O-多糖结构完全一致。