大肠杆菌O140 O抗原的结构与基因簇
Structure and gene cluster of the O-antigen of Escherichia coli O140.
作者信息
Perepelov Andrei V, Wang Quan, Senchenkova Sof'ya N, Mei Zhu, Shashkov Alexander S, Wang Lei, Knirel Yuriy A
机构信息
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
出版信息
Carbohydr Res. 2015 Jun 26;411:33-6. doi: 10.1016/j.carres.2015.04.004. Epub 2015 Apr 17.
An acidic O-polysaccharide (O-antigen) was isolated from the lipopolysaccharide of Escherichia coli O140 and studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the branched hexasaccharide repeating unit was established: [Formula: see text]. The O-antigen gene cluster of E. coli O140 was sequenced. The gene functions were tentatively assigned by a comparison with sequences in the available databases and found to be in full agreement with the E. coli O140 polysaccharide structure.
从大肠杆菌O140的脂多糖中分离出一种酸性O-多糖（O-抗原），并通过糖分析以及一维和二维（1）H和（13）C NMR光谱进行研究。确定了以下分支六糖重复单元的结构：[化学式：见正文]。对大肠杆菌O140的O-抗原基因簇进行了测序。通过与现有数据库中的序列进行比较，初步确定了基因功能，发现与大肠杆菌O140多糖结构完全一致。
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