Phebus L A, Clemens J A
Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, Indiana 46285.
Life Sci. 1989;44(19):1335-42. doi: 10.1016/0024-3205(89)90390-1.
Rat striatal extracellular fluid levels of dopamine, serotonin, 3-methoxytyramine (3-MT), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) were measured before, during and after transient, global cerebral ischemia in awake rats using in vivo brain microdialysis. Before ischemia, extracellular levels of dopamine, DOPAC, HVA and 5-HIAA were detectable and consistent from sample to sample. During cerebral ischemia, there was a large increase in extracellular dopamine levels and a decrease in the extracellular levels of DOPAC, HVA, and 5-HIAA. During reperfusion, dopamine levels returned to normal as did those of DOPAC, HVA and 5-HIAA. Dialysate serotonin and 3-methoxytyramine concentrations were below detection limits except for samples collected during ischemia and early reperfusion.
利用体内脑微透析技术，在清醒大鼠短暂性全脑缺血前、缺血期间及缺血后，测定其纹状体细胞外液中多巴胺、5-羟色胺、3-甲氧基酪胺（3-MT）、3,4-二羟基苯乙酸（DOPAC）、高香草酸（HVA）和5-羟吲哚乙酸（5-HIAA）的水平。缺血前，多巴胺、DOPAC、HVA和5-HIAA的细胞外水平可检测到，且样本间一致。脑缺血期间，细胞外多巴胺水平大幅升高，而DOPAC、HVA和5-HIAA的细胞外水平降低。再灌注期间，多巴胺水平以及DOPAC、HVA和5-HIAA的水平均恢复正常。除缺血期间及再灌注早期采集的样本外，透析液中5-羟色胺和3-甲氧基酪胺的浓度低于检测限。
