van der Ley P, Kuipers O, Tommassen J, Lugtenberg B
Department of Molecular Cell Biology, State University, Utrecht, The Netherlands.
Microb Pathog. 1986 Feb;1(1):43-9. doi: 10.1016/0882-4010(86)90030-6.
The accessibility of outer membrane pore protein PhoE to antibody molecules at the cell surface of intact cells of various Enterobacteriaceae was investigated. Significant antibody binding was observed for only two of the nine strains tested. Analysis of the lipopolysaccharide by sodium dodecyl sulphate gel electrophoresis revealed a clear correlation between the presence of an O-antigenic side chain and the inability to bind PhoE protein-specific antibodies. As mutants that lack the O-antigen chain appeared to have acquired the ability to bind antibody, it must be concluded that the presence of O-antigenic chains of lipopolysaccharide prevents binding of antibodies to PhoE protein at the surface of intact cells. The relevance of this conclusion for the potential use of enterobacterial outer membrane pore proteins as vaccine components is discussed.
研究了外膜孔蛋白PhoE在各种肠杆菌科完整细胞表面对抗体分子的可及性。在所测试的9个菌株中，仅观察到2个菌株有明显的抗体结合。通过十二烷基硫酸钠凝胶电泳对脂多糖进行分析，结果显示O抗原侧链的存在与无法结合PhoE蛋白特异性抗体之间存在明显的相关性。由于缺乏O抗原链的突变体似乎获得了结合抗体的能力，因此必须得出结论，脂多糖的O抗原链的存在会阻止抗体与完整细胞表面的PhoE蛋白结合。讨论了这一结论与将肠杆菌外膜孔蛋白用作疫苗成分的潜在用途的相关性。
