Parts Leopold
Department of Molecular Genetics, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Canada.
Yeast. 2014 Jun;31(6):197-205. doi: 10.1002/yea.3010. Epub 2014 May 15.
Yeast has long enjoyed superiority as a genetic model because of its short generation time and ease of generating alleles for genetic analysis. However, recent developments of guided nucleases for genome editing in higher eukaryotes, and funding pressures for translational findings, force all model organism communities to reaffirm and rearticulate the advantages of their chosen creature. Here I examine the utility of budding yeast for understanding the genetic basis of cellular traits, using natural variation as well as classical genetic perturbations, and its future prospects compared to undertaking the work in human cell lines. Will yeast remain central, or will it join the likes of phage as an early model that is no longer widely used to answer the pressing questions?
长期以来，酵母作为一种遗传模型备受青睐，因为其世代时间短，且易于生成用于遗传分析的等位基因。然而，高等真核生物基因组编辑中引导核酸酶的最新进展，以及转化研究成果所面临的资金压力，促使所有模式生物研究群体重新确认并阐明他们所选生物的优势。在这里，我探讨了芽殖酵母在利用自然变异以及经典遗传扰动来理解细胞性状遗传基础方面的实用性，以及与在人类细胞系中开展相关工作相比它的未来前景。酵母会继续占据核心地位，还是会像噬菌体一样，成为一种不再广泛用于解答紧迫问题的早期模型呢？
