Li Yuping, Chen Xi, Ribeiro Artur J, Jensen Eric D, Holmberg Kyle V, Rodriguez-Cabello J Carlos, Aparicio Conrado
Minnesota Dental Research Center for Biomaterials and Biomechanics, Department of Restorative Sciences, School of Dentistry, University of Minnesota, 55455, Minneapolis, MN, USA.
Adv Healthc Mater. 2014 Oct;3(10):1638-47. doi: 10.1002/adhm.201400015. Epub 2014 Apr 2.
Modification of surfaces mimicking unique chemical and physical features of mineralized tissues is of major interest for obtaining biomaterials for replacing and regenerating biological tissues. Here, human salivary statherin-inspired genetically engineered recombinamers (ELRs, HSS) on biomedical surfaces regulates mineralization to form an amorphous-calcium-phosphate (ACP) layer that reproduces the original substrate nanotopography. The HSS-ELRs carry a statherin-derived peptide with high affinity to tooth enamel. They are tethered to nanorough surfaces and mineralized using an enzyme-directed process. A homogeneous layer of ACP-minerals forms on HSS-coated surfaces retaining the original nanotopography of the substrate. In contrast, biomineralization of control surfaces results in uncontrolled growth of minerals. This suggest the statherin-inspired ELRs have ability to induce and control growth of the minerals on the biofunctional surfaces. Likely, the HSS-ELR coating have similar bioactivity to that of statherin in human saliva. The hybrid nanorough surfaces improve adhesion and differentiation of preosteoblasts and show potential for dental and orthopedic implants integration. This method enables the combination and tailoring of nanotopographical and biochemical cues to design functionalized surfaces to investigate and potentially direct the stem cell fate.
模仿矿化组织独特化学和物理特征的表面修饰，对于获取用于替代和再生生物组织的生物材料而言至关重要。在此，受人类唾液富组蛋白启发的基因工程重组体（ELRs，HSS）在生物医学表面上调节矿化过程，以形成一层无定形磷酸钙（ACP）层，该层可重现原始底物的纳米拓扑结构。HSS-ELRs携带一种对牙釉质具有高亲和力的源自富组蛋白的肽。它们被 tethered 到纳米粗糙表面，并通过酶导向过程进行矿化。在涂有HSS的表面上形成一层均匀的ACP-矿物质层，保留了底物的原始纳米拓扑结构。相比之下，对照表面的生物矿化会导致矿物质的无控制生长。这表明受富组蛋白启发的ELRs有能力在生物功能表面上诱导和控制矿物质的生长。很可能，HSS-ELR涂层具有与人类唾液中富组蛋白相似的生物活性。这种混合纳米粗糙表面改善了前成骨细胞的粘附和分化，并显示出在牙科和骨科植入物整合方面的潜力。这种方法能够将纳米拓扑和生化线索进行组合和定制，以设计功能化表面，从而研究并潜在地引导干细胞命运。
原文中“tethered”这个词在提供的中文译文中暂时保留英文形式，因为不太明确其确切含义，需结合更多背景信息准确翻译。
