Minnesota Dental Research Center for Biomaterials and Biomechanics, Department of Restorative Sciences, University of Minnesota School of Dentistry, Minneapolis, MN 55455, USA.
Colloids Surf B Biointerfaces. 2013 Jul 1;107:189-97. doi: 10.1016/j.colsurfb.2013.02.005. Epub 2013 Feb 21.
Functionalization of implants with multiple bioactivities is desired to obtain surfaces with improved biological and clinical performance. Our objective was developing a simple and reliable method to obtain stable multifunctional coatings incorporating different oligopeptides. We co-immobilized on titanium surface oligopeptides of known cooperative bioactivities with a simple and reliable method. Appropriately designed oligopeptides containing either RGD or PHSRN bioactive sequences were mixed and covalently bonded on CPTES-silanized surfaces. Coatings made of only one of the two investigated peptides and coatings with physisorbed oligopeptides were produced and tested as control groups. We performed thorough characterization of the obtained surfaces after each step of the coating preparation and after mechanically challenging the obtained coatings. Fluorescence labeling of RGD and PHSRN peptides with fluorescence probes of different colors enabled the direct visualization of the co-immobilization of the oligopeptides. We proved that the coatings were mechanically stable. The surfaces with co-immobilized RGD and PHSRN peptides significantly improved osteoblasts response in comparison with control surfaces, which assessed the effectiveness of our coating method to bio-activate the implant surfaces. This same simple method can be used to obtain other multi-functional surfaces by co-immobilizing oligopeptides with different targeted bioactivities--cell recruitment and differentiation, biomineral nucleation, antimicrobial activity--and thus, further improving the clinical performance of titanium implants.
将多种生物活性功能化于植入物上，以获得具有改善的生物学和临床性能的表面，这是人们所期望的。我们的目标是开发一种简单可靠的方法，以获得包含不同短肽的稳定多功能涂层。我们采用一种简单可靠的方法，将具有已知协同生物活性的短肽共同固定在钛表面上。将含有 RGD 或 PHSRN 生物活性序列的适当设计的短肽混合并通过 CPTES-硅烷化表面进行共价键合。只使用两种研究肽之一制成的涂层和具有物理吸附短肽的涂层被制作并作为对照组进行测试。在涂层制备的每一步以及在获得的涂层经受机械挑战之后，我们对获得的表面进行了彻底的表征。使用不同颜色荧光探针对 RGD 和 PHSRN 肽进行荧光标记，能够直接观察到短肽的共同固定。我们证明了涂层具有机械稳定性。与对照表面相比，共同固定 RGD 和 PHSRN 肽的表面显著改善了成骨细胞的反应，这证明了我们的涂层方法在生物激活植入物表面方面的有效性。通过共同固定具有不同靶向生物活性的短肽（细胞募集和分化、生物矿化核、抗菌活性），同样可以使用这种简单的方法获得其他多功能表面，从而进一步提高钛植入物的临床性能。
